Improved Cold-resistant Performance in Transgenic Grape (Vitis vinifera L.) Overexpressing Cold-inducible Transcription Factors AtDREB1b
Improved Cold-resistant Performance in Transgenic Grape (Vitis vinifera L.) Overexpressing Cold-inducible Transcription Factors AtDREB1b
复制标题
过表达寒冷诱导转录因子 AtDREB1b 的转基因葡萄 (Vitis vinifera L.) 的抗寒性能得到改善
DOI：
10.21273/hortsci.44.1.35
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发表时间：
2009-02-01
期刊：
影响因子：
1.9
通讯作者：
Han, Zhenhai
中科院分区：
文献类型：
--
作者：
Jin, Wanmei;Dong, Jing;Han, Zhenhai
脱水反应元件结合 (DREB)1b 是拟南芥中的冷诱导转录因子。由花椰菜花叶病毒 35S 启动子驱动的 DREB1b 被基因导入葡萄 Vitis vinifera L. cv. 中。百年无核通过农杆菌介导转化提高其抗寒性，探索新的遗传育种途径以获得抗寒品种。在这项研究中，Southern印迹分析显示DREB1b基因以一到两个拷贝整合到转基因葡萄藤中。 Northern印迹分析显示独立转基因品系3、5、6和7中存在DREB1b转录本。转基因葡萄树的进一步表征证实转基因植物的电解质渗漏电导率和冰点均低于野生型植物。 -4℃8℃冷处理12h后，26％的转基因植株枯萎，其中95％的植株置于22℃～25℃8℃条件下恢复。然而，经过同样的处理，98%的非转基因植物枯萎，只有2%恢复。我们的结果得出这样的结论：转基因葡萄中DREB1b的活性可以显着提高其对冷胁迫的抵抗力。
Dehydration response element binding (DREB)1b is a cold-inducible transcrip- tion factor in Arabidopsis thaliana. DREB1b driven by cauliflower mosaic virus 35S promoter was genetically introduced into grape Vitis vinifera L. cv. Centennial Seedless through Agrobacterium-mediated transformation for improving its cold resistance and exploring new genetic breeding approaches to obtain cold-resistant cultivars. In this study,SouthernblotanalysisshowedtheDREB1bgenewasintegratedintothetransgenic grapevines with one to two copies. Northern blot analysis showed the presence of DREB1b transcripts in the independent transgenic lines 3, 5, 6, and 7. Further characterization of transgenic grapevines confirmed that both electrolyte leakage conductivity and the freezing point of the transgenic plants were lower than those of wild-type plants. After the cold treatment at -4 8C for 12 h, 26% of transgenic plants wilted among which 95% plants recovered once being placed under the condition of temperature 22 to 25 8C. However, subjected to the same treatment, 98% of non- transgenic plants wilted and only 2% recovered. Our results lead to the conclusion that activity of DREB1b in the transgenic grape could significantly improve its resistance to cold stress.