MOLECULAR-CLONING OF 2 PIGMENT-DISPERSING HORMONE (PDH) PRECURSORS IN THE BLUE-CRAB CALLINECTES-SAPIDUS REVEALS A NOVEL MEMBER OF THE PDH NEUROPEPTIDE FAMILY
MOLECULAR-CLONING OF 2 PIGMENT-DISPERSING HORMONE (PDH) PRECURSORS IN THE BLUE-CRAB CALLINECTES-SAPIDUS REVEALS A NOVEL MEMBER OF THE PDH NEUROPEPTIDE FAMILY
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DOI：
10.1006/bbrc.1994.2680
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发表时间：
1994-11-30
影响因子：
3.1
通讯作者：
RAO, KR
中科院分区：
文献类型：
--
作者：
KLEIN, JM;MOHRHERR, CJ;RAO, KR
建立了青蟹眼柄神经节cDNA文库。筛选导致分离出一个克隆（497 bp，不包括poly（A）尾），其编码先前在几种甲壳类动物中发现的β-PDH。该基因与从海岸Carcinus maenas眼柄cDNA文库中获得的一个克隆具有较高的同源性，表明两种蟹的系统发育关系较近。第二个克隆（414 bp，不包括poly（A）尾）编码一种新的β-PDH类似物，其在螃蟹生物测定中显示出低400倍的效力。两个cDNA编码前原肽的234 bp的开放阅读框，由信号肽、PDH前体相关肽和PDH序列组成。(C)1994年出版社出版。
A cDNA library was established from the eyestalk ganglia of the blue crab Callinectes sapidus. Screening resulted in the isolation of a clone (497 bp excluding poly(A) tail) which encodes a beta-PDH previously found in several crustacean species. It displays high sequence similarity with a clone isolated from an eyestalk cDNA library of the shore crab Carcinus maenas, indicating the close phylogenetic relationship of both species. A second clone (414 bp exclusive of the poly(A) tail) encodes a novel beta-PDH analog which displays 400-fold less potency in crab bioassays. Both cDNAs encode open reading frames of 234 bp for the prepropeptides, consisting of signal peptides, PDH-precursor-related peptides, and PDH sequences. (C) 1994 Academic Press, Inc.