Highly Sensitive Electrochemiluminescence Detection of Mercury(II) Ions Based on DNA‐Linked Luminol‐Au NPs Superstructure
Highly Sensitive Electrochemiluminescence Detection of Mercury(II) Ions Based on DNA‐Linked Luminol‐Au NPs Superstructure
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DOI：
10.1002/elan.201300628
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发表时间：
2014-04
期刊：
影响因子：
3
通讯作者：
Jun Wan;G. Yin;Xiuju Ma;Lingbao Xing;Xiliang Luo
中科院分区：
文献类型：
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作者：
Jun Wan;G. Yin;Xiuju Ma;Lingbao Xing;Xiliang Luo
建立了一种高灵敏度的汞离子电化学发光（ECL）检测方法.基于强而稳定的胸腺嘧啶错配络合物配位化学，汞（II）离子可以特异性地结合到设计的DNA链，导致互补DNA链的释放。释放出的DNA链被修饰有特异性DNA的磁珠捕获，然后通过形成DNA连接的鲁米诺-金纳米颗粒（NPs）超结构，开发了一种针对汞离子的特异性ECL体系。以3-氨丙基-三乙氧基硅烷为增强剂，该体系可在2.0×10−10 mol L−1 ~ 2.0×10−8 M范围内对Hg 2+离子进行线性检测，检测限低至1.05×10−10 M（3σ）。该体系对Hg ~（2+）具有高度的特异性，不受共存金属离子的干扰，可用于真实的样品的分析。
A novel highly sensitive electrochemiluminescence (ECL) detection protocol for mercury(II) ions was developed. Based on the strong and stable thyminethymine mismatches complexes coordination chemistry, mercury(II) ions can specifically bind to a designed DNA strand, leading to the release of the complimentary DNA strand. The released DNA strand was then captured by magnetic beads modified with specific DNA, and then through the formation of DNA-linked luminol-Au nanoparticles (NPs) superstructure, a specific ECL system for mercury(II) ions was developed. Using 3-aminopropyl-triethoxysilane as an effective enhancer, the ECL system can detect Hg2+ ion within a linear range from 2.0×10−10 mol L−1 to 2.0×10−8 M, with a detection limit as low as 1.05×10−10 M (3σ). Moreover, this ECL system is highly specific for Hg2+, without interference from other commonly coexisted metal ions, and it can be used for the analysis of real samples.