Selective breeding and selection mapping using a novel wild-derived heterogeneous stock of mice revealed two closely-linked loci for tameness.
Selective breeding and selection mapping using a novel wild-derived heterogeneous stock of mice revealed two closely-linked loci for tameness.
复制标题
DOI：
10.1038/s41598-017-04869-1
复制
发表时间：
2017-07-04
影响因子：
4.6
通讯作者：
Koide T
中科院分区：
文献类型：
--
作者：
Matsumoto Y;Goto T;Nishino J;Nakaoka H;Tanave A;Takano-Shimizu T;Mott RF;Koide T
驯服是驯化的一个主要行为因素，可以分为两个潜在的组成部分：接近人类的动机（主动驯服）和不愿意避免人类（被动驯服）。我们通过选择育种、选择作图和关联分析确定了主动驯服的遗传位点。在以前的工作中，使用实验室和野生小鼠品系，我们发现，实验室品系主要是选择被动驯服，但不是主动驯服在他们的驯化。为了确定与主动驯服相关的遗传区域，我们采用了9代以上的选择性育种接触，一个与主动驯服密切相关的行为参数。成功选择育种的先决条件是目标群体中的高遗传变异，因此我们建立并使用了一种新的资源，来自8个野生品系的野生衍生异质原种（WHS）小鼠。这些小鼠具有其他远系繁殖小鼠群体中不存在的遗传变异。WHS小鼠的选择性繁殖通过世代增加了接触水平。选择映射被应用到选定的人口使用模拟的基础上的非选择模型和推断单倍型数据来自单核苷酸多态性。我们在11号染色体上发现了一个用于选择的基因组标记，该标记包含两个紧密连锁的基因座。
Tameness is a major behavioral factor for domestication, and can be divided into two potential components: motivation to approach humans (active tameness) and reluctance to avoid humans (passive tameness). We identified genetic loci for active tameness through selective breeding, selection mapping, and association analysis. In previous work using laboratory and wild mouse strains, we found that laboratory strains were predominantly selected for passive tameness but not active tameness during their domestication. To identify genetic regions associated with active tameness, we applied selective breeding over 9 generations for contacting, a behavioural parameter strongly associated with active tameness. The prerequisite for successful selective breeding is high genetic variation in the target population, so we established and used a novel resource, wild-derived heterogeneous stock (WHS) mice from eight wild strains. The mice had genetic variations not present in other outbred mouse populations. Selective breeding of the WHS mice increased the contacting level through the generations. Selection mapping was applied to the selected population using a simulation based on a non-selection model and inferred haplotype data derived from single-nucleotide polymorphisms. We found a genomic signature for selection on chromosome 11 containing two closely linked loci.