Non-specific phospholipase C5 and diacylglycerol promote lateral root development under mild salt stress in Arabidopsis
Non-specific phospholipase C5 and diacylglycerol promote lateral root development under mild salt stress in Arabidopsis
复制标题
DOI：
10.1111/pce.12334
复制
发表时间：
2014-09-01
影响因子：
7.3
通讯作者：
Wang, Xuemin
中科院分区：
文献类型：
--
作者：
Peters, Carlotta;Kim, Sang-Chul;Wang, Xuemin
发展强大的根系对于植物生存和争夺土壤资源至关重要。在此，我们报道非特异性磷脂酶 C5 (NPC5) 及其衍生的脂质介质二酰甘油 (DAG) 在拟南芥盐胁迫期间介导侧根 (LR) 发育。 T-DNA 敲除突变体 npc5-1 在轻度 NaCl 胁迫下几乎不产生 LR，而 NPC5 的过度表达则增加了 LR 数量。 npc5-1 的根含有比野生型更低水平的 DAG，而 NPC5 过表达者则表现出 DAG 水平增加。在 NaCl 胁迫下，应用 DAG（而非磷脂酸）将 npc5-1 的 LR 生长完全恢复至野生型。经 NaCl 处理的拟南芥幼苗中 NPC5 表达显着诱导。 Npc5-1 对生长素介导的根生长的反应低于野生型。这些结果表明 NPC5 介导 LR 发育以响应盐胁迫，并表明 DAG 在胁迫信号传导中充当脂质介质。压力下的生长和模式形成。
Developing a robust root system is crucial to plant survival and competition for soil resources. Here we report that the non-specific phospholipase C5 (NPC5) and its derived lipid mediator diacylglycerol (DAG) mediate lateral root (LR) development during salt stress in Arabidopsis thaliana. T-DNA knockout mutant npc5-1 produced few to no LR under mild NaCl stress, whereas overexpression of NPC5 increased LR number. Roots of npc5-1 contained a lower level of DAG than wild type, whereas NPC5 overexpressor exhibited an increase in DAG level. Application of DAG, but not phosphatidic acid, fully restored LR growth of npc5-1 to that of wild type under NaCl stress. NPC5 expression was significantly induced in Arabidopsis seedlings treated with NaCl. Npc5-1 was less responsive to auxin-mediated root growth than the wild type. These results indicate that NPC5 mediates LR development in response to salt stress and suggest that DAG functions as a lipid mediator in the stress signalling. growth and pattern formation under stress.