Delineation of Borrelia burgdorferi p66 sequences required for integrin αIIbβ3 recognition
Delineation of Borrelia burgdorferi p66 sequences required for integrin αIIbβ3 recognition
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DOI：
10.1128/iai.69.5.3455-3459.2001
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发表时间：
2001-05-01
影响因子：
3.1
通讯作者：
Coburn, J
中科院分区：
文献类型：
--
作者：
Defoe, G;Coburn, J
莱姆病病原体伯氏疏螺旋体的外膜蛋白p66已被鉴定为β（3）链整联蛋白的候选配体。为了鉴定整联蛋白识别所需的p66部分，测试麦芽糖结合蛋白与p66片段的融合物与整联蛋白α（II B）β（3）的结合，并测试衍生自p66氨基酸序列的合成肽抑制B的能力。burgdorferi附着于相同的整联蛋白。这些数据确定了p66的两个不连续的片段，它们对α（IIb）β（3）识别很重要，这表明，与其他整合素配体一样，p66的三级结构对受体识别很重要。
The outer membrane protein p66 of the Lyme disease agent, Borrelia burgdorferi, has been identified as a candidate ligand for beta (3)-chain integrins. To identify portions of p66 required for integrin recognition, fusions of maltose-binding protein to fragments of p66 were tested for binding to integrin alpha (IIb)beta (3) and synthetic peptides derived from the p66 amino acid sequence were tested for the ability to inhibit B. burgdorferi attachment to the same integrin. The data identify two noncontiguous segments of p66 that are important for alpha (IIb)beta (3) recognition, suggesting that, as is true for other integrin ligands, the tertiary structure of p66 is important for receptor recognition.