Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch
Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch
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DOI：
10.1016/j.jbiomech.2019.109371
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发表时间：
2019-11-11
影响因子：
2.4
通讯作者：
Matsumoto, Takeo
中科院分区：
文献类型：
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作者：
Maeda, Eijiro;Atsumi, Yoshinori;Matsumoto, Takeo
多能干细胞被认为是再生医学中的关键材料，了解骨髓来源细胞分化潜能的异质性对于成功的再生组织修复具有重要意义。因此，本研究旨在研究体外循环拉伸对采集后的天然骨髓来源细胞分化的调节作用。取小鼠双后肢股骨获取骨髓源性细胞，分为5类：变形细胞、圆形细胞、梭形细胞、星形细胞等。将细胞接种在硅胶制成的牵伸室中，以1 Hz的频率以10%的幅度循环拉伸7d进行细胞形态分析，3d分析成骨(骨钙素)、血管平滑肌(α-平滑肌肌动蛋白和肌球蛋白重链)和神经源性(神经丝)分化标志物蛋白的表达。在不考虑细胞形态差异的情况下，应用10%的周期拉伸抑制了成骨和神经分化，但促进了平滑肌的分化。近距离观察发现，循环拉伸对圆形细胞的影响最大(4种标志物蛋白的表达均显著上调或下调)，而卵母细胞和梭形细胞仅受循环拉伸对血管平滑肌和/或神经源性分化的影响。据作者所知，这是第一个报道骨髓来源细胞机械应变的命运决定标准与形状相关的差异的研究。(C)2019爱思唯尔有限公司。保留所有权利。
Multipotent stem cells are considered as a key material in regenerative medicine, and the understanding of the heterogeneity in the differentiation potentials of bone marrow-derived cells is important in the successful regenerative tissue repair. Therefore, the present study has been performed to investigate how the differentiation of post-harvest, native bone marrow-derived cells is regulated by cyclic stretch in vitro. Bone marrow-derived cells were obtained from mouse femur of both hind limbs and categorized into the following five categories: amebocytes, round cells, spindle cells, stellate cells and others. The cells were seeded on a silicone-made stretch chamber, and subjected to cyclic stretch with an amplitude of 10% at a frequency of 1 Hz for 7 days for cell shape analysis and for 3 days for the analysis of the expression of marker proteins of osteogenic (osteocalcin), vascular smooth muscle (alpha-smooth muscle actin and smooth muscle myosin heavy chain) and neurogenic (neurofilament) differentiation. When disregarding the differences in the cell shapes, there was an overall trend that the application of 10% cyclic stretch inhibited osteogenic and neurogenic differentiation, but enhanced smooth muscle differentiation. Close examinations revealed that round cells were influenced the most by cyclic stretch (significant up-or down-regulation in all the four marker protein expressions) while amebocytes and spindle cells were only influenced by cyclic stretch for vascular smooth muscle and/or neurogenic differentiation. As far as the authors know, this is the first study reporting the shape-related differences in the fate decision criteria for mechanical strain in bone marrow-derived cells. (C) 2019 Elsevier Ltd. All rights reserved.