Biomaterial adherent macrophage apoptosis is increased by hydrophilic and anionic substrates in vivo
Biomaterial adherent macrophage apoptosis is increased by hydrophilic and anionic substrates in vivo
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DOI：
10.1073/pnas.162124199
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发表时间：
2002-08-06
影响因子：
11.1
通讯作者：
Anderson, JM
中科院分区：
文献类型：
--
作者：
Brodbeck, WG;Patel, J;Anderson, JM
在体内的大鼠笼植入系统中，通过限制单核细胞粘附和巨噬细胞融合到异物巨细胞中，同时诱导粘附巨噬细胞凋亡，来确定防止植入生物医学装置和假体失败的潜在表面化学物质。采用疏水、亲水性、阴离子和阳离子表面进行植入。对材料周围渗出物的分析显示，在存在的细胞类型或水平的表面之间没有差异。相反，与聚对苯二甲酸乙二醇酯基表面相比，阴离子表面和亲水表面的贴壁细胞凋亡比例显著增加（分别为46 +/- 3.7%和57 +/- 5.0%）。此外，亲水性和阴离子底物降低了单核细胞/巨噬细胞的粘附和融合率。这些研究表明，生物材料贴壁细胞在体内经历了材料依赖性的凋亡，使潜在有害的巨噬细胞失去功能，而植入物周围的环境不受影响。
An in vivo rat cage implant system was used to identify potential surface chemistries that prevent failure of implanted biomedical devices and prostheses by limiting monocyte adhesion and macrophage fusion into foreign-body giant cells while inducing adherent-macrophage apoptosis. Hydrophobic, hydrophilic, anionic, and cationic surfaces were used for implantation. Analysis of the exudate surrounding the materials revealed no differences between surfaces in the types or levels of cells present. Conversely, the proportion of adherent cells undergoing apoptosis was increased significantly on anionic and hydrophilic surfaces (46 +/- 3.7 and 57 +/- 5.0%, respectively) when compared with the polyethylene terephthalate base surface. Additionally, hydrophilic and anionic substrates provided decreased rates of monocyte/macrophage adhesion and fusion. These studies demonstrate that biomaterial-adherent cells undergo material-dependent apoptosis in vivo, rendering potentially harmful macrophages nonfunctional while the surrounding environment of the implant remains unaffected.