Characterization of Mhc genes in a multigenerational family of ring-necked pheasants.
Characterization of Mhc genes in a multigenerational family of ring-necked pheasants.
复制标题
环颈雉多代家系 Mhc 基因的特征。
DOI：
10.1007/bf00176673
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发表时间：
1996
期刊：
影响因子：
3.2
通讯作者：
Miller,MM
中科院分区：
文献类型：
--
作者：
Jarvi,SI;Goto,RM;Briles,WE;Miller,MM
关于不同分类群中鸟类的主要组织相容性（Mhc）基因以及Mhc基因在不同进化或栖息地的鸟类物种中的组织方式知之甚少。然而，我们似乎可以从鸟类身上学到很多关于这种复杂的多态基因的进化、组织和功能的知识。在这项研究中，鸡的近亲，环颈雉（Phasianus colchicus），Mhc B-G基因的存在和组织进行了检查。鸡B-G探针在Southern杂交中显示的限制性片段的模式和野鸡红细胞多肽的模式在免疫印迹中显示的抗鸡B-G多肽的抗血清提供了遗传、分子和生物化学数据，证实了早期血清学证据，即在野鸡中存在B-G基因，因此，在至少第二种鸟类中存在B-G基因家族。在本研究的小实验群体中，通过限制性片段图谱，可以很容易地检测到雉鸡B-G基因家族所表现出的高度多态性。进一步的证据被发现的pheasantMhc类I和II类基因的组织成遗传上独立的集群。这些基因簇是否与鸡中的BandRfp-Y系统完全相似，或者是否在野鸡中发现了另一种MHC基因组织，仍有待确定。
Little is known about the major histocompatibility (Mhc) genes of birds in different taxonomic groups or about howMhcgenes may be organized in avian species divergent by evolution or habitat. Yet it seems likely that much might be learned from birds about the evolution, organization, and function of this intricate complex of polymorphic genes. In this study a close relative of the chicken, the ring-necked pheasant (Phasianus colchicus), was examined for the presence and organization ofMhc B-Ggenes. The patterns of restriction fragments revealed by chickenB-Gprobes in Southern hybridizations and the patterns of pheasant erythrocyte polypeptides revealed in immunoblots by antisera raised against chicken B-G polypeptides provide genetic, molecular, and biochemical data confirming earlier serological evidence for the presence ofB-Ggenes in the pheasant, and hence, the presence of a family ofB-Ggenes in at least a second species of birds. The high polymorphism exhibited by the pheasantB-Ggene family allowed genetic differences among individuals within the small experimental population in this study to be detected easily by restriction fragment patterns. Further evidence was found for the organization of the pheasantMhcclass I and class II genes into genetically independent clusters. Whether these gene clusters are fully comparable to theBandRfp-Ysystems in the chicken or whether yet another organization ofMhcgenes has been encountered in the pheasant remains to be determined.