PHOSPHORYLATION OF NITROGEN REGULATOR-I (NRI) OF ESCHERICHIA-COLI
PHOSPHORYLATION OF NITROGEN REGULATOR-I (NRI) OF ESCHERICHIA-COLI
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DOI：
10.1073/pnas.85.23.8919
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发表时间：
1988-12-01
影响因子：
11.1
通讯作者：
MAGASANIK, B
中科院分区：
文献类型：
--
作者：
WEISS, V;MAGASANIK, B
It has previously been shown that phosphorylated nitrogen regulator I (NRi-phosphate) is the activator responsible for increasing the transcription of glnA, the structural gene for glutamine synthetase, and that NRII catalyzes the transfer of the .gamma.-phosphate of ATP to NRI. We have now shown that the reaction of ATP with NRII results in the reversible transfer of the .gamma.-phosphate of ATP to a histidine residue of NRII. In turn, NRII-phosphate transfer its phosphate reversibly to an aspartic residue of NRI-phosphate is hydrolyzed to NRI and inorganic phosphate in a divalent cation-requiring autocatalytic reaction.
