LAMININ-A CHAIN - EXPRESSION DURING DROSOPHILA DEVELOPMENT AND GENOMIC SEQUENCE
LAMININ-A CHAIN - EXPRESSION DURING DROSOPHILA DEVELOPMENT AND GENOMIC SEQUENCE
复制标题
DOI：
10.1002/j.1460-2075.1992.tb05553.x
复制
发表时间：
1992-12-01
期刊：
影响因子：
11.4
通讯作者：
FESSLER, JH
中科院分区：
文献类型：
--
作者：
KUSCHEGULLBERG, M;GARRISON, K;FESSLER, JH
果蝇层粘连蛋白A链基因全长14kb，编码一个由15个外显子3712个氨基酸组成的开放阅读框。总体而言，这种A链与脊椎动物的对应链相似，特别是在其N-末端和C-末端的球状结构域上，但形成层粘连蛋白A短臂的序列非常不同，而且更大。层粘连蛋白信息出现在新形成的中胚层，随后在血细胞中显著表达，血细胞也合成基底膜IV型胶原。果蝇基底膜的组成随着发育而变化。一种新的融合RNA探针方法表明，层粘连蛋白mRNAs的发育增加主要与形态发生阶段有关，并先于生长过程中强烈表达的IV型胶原蛋白。A：B1：B2mRNAs的比例在胚胎发育过程中变化不大，A链的mRNA量少于B链。用抗体对胚胎进行染色，证实并扩展了原位杂交提供的信息。该A链G-亚区的同源物存在于集聚蛋白、Perlecan、层粘连蛋白和性类固醇结合蛋白的相互作用区域，可能参与蛋白质关联。
A Drosophila laminin A chain gene was characterized as a 14 kb genomic nucleotide sequence which encodes an open reading frame of 3712 amino acids in 15 exons. Overall, this A chain is similar to its vertebrate counterparts, especially in its N- and C-terminal globular domains, but the sequence that forms the laminin A short arm is quite different and larger. Laminin messages appear in newly formed mesoderm and are later prominently expressed in hemocytes, which also synthesize basement membrane collagen IV. The composition of Drosophila basement membranes changes with development. A novel method of tandemly fused RNA probes showed that developmental increases of laminin mRNAs were primarily associated with periods of morphogenesis, and preceded those of collagen IV, a protein strongly expressed during growth. The ratio of A:B1:B2 mRNAs varied little during embryogenesis, with less mRNA for A than B chains. Staining of embryos with antibodies confirmed and extended the information provided by in situ hybridization. Homologs of the G-subdomains of this A chain, which occur in interacting regions of agrin, perlecan, laminin and sex steroid binding protein, may be involved in protein associations.