Microfluidic enrichment of a target cell type from a heterogenous suspension by adhesion-based negative selection
Microfluidic enrichment of a target cell type from a heterogenous suspension by adhesion-based negative selection
复制标题
DOI：
10.1039/b906768j
复制
发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Murthy, Shashi K.
中科院分区：
文献类型：
--
作者：
Green, James V.;Murthy, Shashi K.
当这些细胞的表面标志物不完全已知时，在异质悬浮液中富集或分离选定类型的细胞是具有挑战性的。在这里，我们提出了一个3阶段的肽涂层微流控通道，可以回收少量的脂肪来源的干细胞（ADSCs）从异质性悬浮液的非靶细胞类型的负选择耗尽。
The enrichment or isolation of a selected type of cells in a heterogeneous suspension is challenging when the surface markers of these cells are not completely known. Here, we present a 3-stage arrangement of peptide-coated microfluidic channels that can recover a small number of adipose-derived stem cells (ADSCs) from a heterogenous suspension by negative selection depletion of the non-target cell types.