COMPARISON OF THE FREE AND DNA-COMPLEXED FORMS OF THE DNA-BINDING DOMAIN FROM C-MYB
COMPARISON OF THE FREE AND DNA-COMPLEXED FORMS OF THE DNA-BINDING DOMAIN FROM C-MYB
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DOI：
10.1038/nsb0495-309
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发表时间：
1995-04-01
期刊：
影响因子：
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通讯作者：
NISHIMURA, Y
中科院分区：
文献类型：
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作者：
OGATA, K;MORIKAWA, S;NISHIMURA, Y
c-Myb 的 DNA 结合结构域由三个不完美的串联重复序列（R1、R2 和 R3）组成。三个重复具有相似的整体架构。每个都包含螺旋-转角-螺旋变异基序。每个重复中的三个保守色氨酸参与形成疏水核心。三个重复结构的比较表明，在热不稳定的R2的疏水核心中发现了空腔。与 DNA 络合时，R2 和 R3 的方向通过紧密结合而固定，并且它们的构象略有变化。 R1 的化学位移没有发生显着变化，与其与 DNA 的松散相互作用一致。
The DNA-binding domain of c-Myb consists of three imperfect tandem repeats (R1, R2 and R3). The three repeats have similar overall architectures. each containing a helix-turn-helix variation motif. The three conserved tryptophans in each repeat participate in forming a hydrophobic core. Comparison of the three repeat structures indicated that cavities are found in the hydrophobic core of R2, which is thermally unstable. On complexation with DNA, the orientations of R2 and R3 are fixed by tight binding and their conformations;ire slightly changed. No significant changes occur in the chemical shifts of R1 consistent with its loose interaction with DNA.