MOTILITY, CELL-SHAPE, AND LOCOMOTION OF NEUTROPHIL GRANULOCYTES
MOTILITY, CELL-SHAPE, AND LOCOMOTION OF NEUTROPHIL GRANULOCYTES
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DOI：
10.1002/cm.970030105
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发表时间：
1983-01-01
影响因子：
--
通讯作者：
KELLER, HU
中科院分区：
文献类型：
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作者：
KELLER, HU
在附着于固体基质的细胞中，不能可靠地评估趋化因子对能动性器官的激活，因为过度粘附可以完全消除能动性。然而，为了分析和理解化学动力学反应，有必要量化能动装置的激活。建立了形态学标准来量化非粘附（漂浮）中性粒细胞的运动性，并预测在有限粘附条件下的运动反应。悬浮液中进行爬行样运动的嗜中性粒细胞（极化细胞）的比例与低至最佳浓度的f-Met-Leu-Phe下的刺激运动非常密切相关，即，在有限的粘附条件下。在超最佳浓度的f-Met-Leu-Phe减少运动也有形态学相关性。主要特征是进行爬行样运动的中性粒细胞比例降低，相应出现可运动但在悬浮液中不极化且不在基质上移动的细胞。还观察到中性粒细胞长度和有尾和无尾极化中性粒细胞比例的浓度依赖性变化。在与基质有限接触的条件下，嗜中性粒细胞的运动潜力可以根据其运动行为，特别是悬浮液中显示爬行样运动的细胞比例来预测。结合测量的粘附力的程序应该允许一个更完整的分析，调节的化学动力学反应。
Activation of the motile apparatus by chemokinetic factors cannot be reliably assessed in cells that are attached to a solid substratum because motility can be totally abolished by excessive adhesion. It is however, necessary to quantify the activation of the motile apparatus in order to analyze and understand chemokinetic responses. Morphological criteria was established to quantify motility in nonadherent (floating) neutrophils and to predict the locomotor response under conditions of limited adhesion. The proportion of neutrophils performing crawling-like movements (polarized cells) in suspension correlates very closely with stimulated locomotion at low to optimal concentration of f-Met-Leu-Phe, i.e., under conditions of limited adhesion. Reduced locomotion at supraoptimal concentrations of f-Met-Leu-Phe also has morphological correlates. The major feature is the decrease in the proportion of neutrophils performing crawling-like movements and the corresponding appearance of cells that are motile but not polarized in suspension and that do not locomote on the substratum. Concentration-dependent changes in neutrophil length and in the proportion of polarized neutrophils with and without tail were also observed. The locomotor potential of neutrophils under conditions of limited contact with the substratum can be predicted on the basis of their motile behavior, in particular, the proportion of cells showing crawling-like movements, in suspension. In combination with measurements of adhesion the procedure should permit a more complete analysis of the regulation of chemokinetic responses.