Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis.
Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis.
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转录因子 RFX2 是小鼠精子发生的关键调节因子
DOI：
10.1038/srep20435
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发表时间：
2016-02-08
影响因子：
4.6
通讯作者：
Han C
中科院分区：
文献类型：
--
作者：
Wu Y;Hu X;Li Z;Wang M;Li S;Wang X;Lin X;Liao S;Zhang Z;Feng X;Wang S;Cui X;Wang Y;Gao F;Hess RA;Han C
调节因子X（RFX）家族的转录因子是至关重要的纤毛发生在整个进化。在小鼠中，Rfx 1 -4在产生鞭毛精子的睾丸中高度表达，但这些因子在精子发生中的功能尚不清楚。在这里，我们报告的Rfx 2基因敲除小鼠的生产和表征。雄性基因敲除小鼠由于在早期圆形精子细胞阶段精子发生受阻而不育。Rfx 2-null圆形精子细胞脱离曲细精管，形成大的多核巨细胞，发生凋亡。在突变体中，鞭毛的形成在其最早阶段被抑制。RNA-seq分析鉴定出大量纤毛相关基因和睾丸特异性基因受RFX 2调控。这些基因中的许多是RFX 2的直接靶标，如染色质免疫沉淀-PCR测定所揭示的。这些发现表明RFX 2是小鼠生精细胞减数分裂后发育的关键调节因子。
The regulatory factor X (RFX) family of transcription factors is crucial for ciliogenesis throughout evolution. In mice, Rfx1-4 are highly expressed in the testis where flagellated sperm are produced, but the functions of these factors in spermatogenesis remain unknown. Here, we report the production and characterization of the Rfx2 knockout mice. The male knockout mice were sterile due to the arrest of spermatogenesis at an early round spermatid step. The Rfx2-null round spermatids detached from the seminiferous tubules, forming large multinucleated giant cells that underwent apoptosis. In the mutants, formation of the flagellum was inhibited at its earliest stage. RNA-seq analysis identified a large number of cilia-related genes and testis-specific genes that were regulated by RFX2. Many of these genes were direct targets of RFX2, as revealed by chromatin immunoprecipitation-PCR assays. These findings indicate that RFX2 is a key regulator of the post-meiotic development of mouse spermatogenic cells.