Surfactant aided biodegradation of NAPLs by Burkholderia multivorans: comparison between Triton X-100 and rhamnolipid JBR-515.
Surfactant aided biodegradation of NAPLs by Burkholderia multivorans: comparison between Triton X-100 and rhamnolipid JBR-515.
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DOI：
10.1016/j.colsurfb.2012.08.064
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发表时间：
2013-02
期刊：
影响因子：
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通讯作者：
Sagarika Mohanty;S. Mukherji
中科院分区：
文献类型：
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作者：
Sagarika Mohanty;S. Mukherji
化学表面活性剂和生物表面活性剂已被有效地用于石油烃的降解。已知化学表面活性剂有效地增强生物降解，而生物表面活性剂的活性也相当，并且它们具有可生物降解的额外优点。然而，化学表面活性剂和生物表面活性剂的作用模式可能会有所不同。通过研究萘降解菌Burkholderia multivorans（NG 1）对非水相模拟液体（NAPLs）的表面活性剂辅助降解过程的影响因素，确定Triton X-100和鼠李糖脂JBR-515的作用方式。进行乳化研究、生长速率和降解速率研究，并与细胞表面性质（包括表面疏水性、细胞表面电荷和细胞表面官能团）的改变相关。Triton X-100和JBR-515表现出不同的NAPL摄取模式。Triton X-100通过乳化提高生物利用度，并支持B对模型NAPL的直接界面摄取。多噬菌体（NG 1）。相反，生物表面活性剂鼠李糖脂JBR-515没有表现出NAPL的乳化和通过胶束增溶增强的生物利用度。NAPL组合物影响细胞表面性质的改变。对于这两种表面活性剂，表面活性剂浓度的增加增加了从NAPL的脂肪烃的利用率。
Both chemical surfactants and biosurfactants have been effectively used for the degradation of petroleum hydrocarbons. Chemical surfactants are known to enhance biodegradation effectively while activity of biosurfactants is also comparable and they have the additional advantage of being biodegradable. However, the mode of action of chemical surfactants and biosurfactants may vary. This work was conducted to determine the mode of action of Triton X-100 and rhamnolipid JBR-515 by exploring the factors affecting the process of surfactant aided biodegradation of model non aqueous phase liquids (NAPLs) by a naphthalene degrader, Burkholderia multivorans (NG1). Emulsification studies, growth rate and degradation rate studies were conducted and correlated with alteration in cell surface properties including surface hydrophobicity, cell surface charge and cell surface functional groups. Triton X-100 and JBR-515 demonstrated distinct mode of uptake of NAPLs. Triton X-100 enhanced bioavailability by emulsification and supported direct interfacial uptake of model NAPLs by B. multivorans (NG1). Conversely, the biosurfactant rhamnolipid JBR-515 did not demonstrate emulsification of NAPLs and enhanced bioavailability through micellar solubilization. NAPL composition influenced the alteration in the cell surface properties. For both the surfactants, increase in surfactant concentration increased the rate of utilization of aliphatic hydrocarbons from the NAPLs.