Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries
Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries
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DOI：
10.1016/s0378-1135(00)00188-7
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发表时间：
2000-06-01
影响因子：
3.3
通讯作者：
Vecht, U
中科院分区：
文献类型：
--
作者：
Wisselink, HJ;Smith, HE;Vecht, U
猪链球菌菌株（n=411），从欧洲7个国家的病猪分离，血清型1至28使用特异性抗血清，并进行了表型的基础上，他们的溶菌酶释放蛋白（MRP）和胞外因子蛋白（EF）的生产。总体而言，S.猪流感血清型2似乎最普遍（32%），其次是血清型9（20%）和血清型1（12%）。血清型2最常在法国、意大利和西班牙分离，而血清型9最常在比利时、荷兰和德国分离。在联合王国，血清型1和14是最常见的分离。高比例的S。从与猪链球菌感染相关的组织如脑、浆膜、关节、心脏和器官中分离的猪链球菌血清型1、2、1/2和14株表达EF蛋白，表明在这些血清型中EF的表达可能与毒力相关。相反，从与S.猪感染不产生EF。这些结果有力地表明，在血清型7和9 EF的表达是不相关的毒力。80%以上的S.猪血清型9菌株产生MRP* 蛋白，其为136 kDa MRP的高分子变体。MRP* 在血清9型菌株中的表达可能与毒力相关。(C)2000 Elsevier Science B. V.保留所有权利。
Streptococcus suis strains (n=411), isolated from diseased pigs in seven European countries were serotyped using specific antisera against serotype 1 to 28, and were phenotyped on the basis of their muramidase-released-protein (MRP) and extracellular-factor protein (EF) production. Overall, S. suis serotype 2 appeared to be most prevalent (32%), followed by serotype 9 (20%) and serotype 1 (12%). Serotype 2 was most frequently isolated in France, Italy and Spain, whereas serotype 9 was most frequently isolated in Belgium, The Netherlands and Germany. In the United Kingdom serotypes 1 and 14 were most frequently isolated. High percentages of S. suis serotype 1, 2, 1/2 and 14 strains, isolated from tissues associated with S, suis infections such as brain, serosa, joint, heart and organs expressed the EF-protein, indicating that in these serotypes expression of EF is likely to be associated with virulence. In contrast, strains belonging to serotype 7 and 9, isolated from tissues associated with S. suis infections did not produce EF. These results strongly suggest that in the serotypes 7 and 9 EF expression is not related to virulence. More than 80% of the S. suis serotype 9 strains produced an MRP* protein, a high molecular variant of the 136 kDa MRP. Expression of MRP* in serotype 9 strains is possibly associated with virulence. (C) 2000 Elsevier Science B.V. All rights reserved.