The synthetic killer peptide KP impairs Candida albicans biofilm in vitro.
The synthetic killer peptide KP impairs Candida albicans biofilm in vitro.
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DOI：
10.1371/journal.pone.0181278
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Peppoloni S
中科院分区：
文献类型：
--
作者：
Paulone S;Ardizzoni A;Tavanti A;Piccinelli S;Rizzato C;Lupetti A;Colombari B;Pericolini E;Polonelli L;Magliani W;Conti S;Posteraro B;Cermelli C;Blasi E;Peppoloni S
白色念珠菌是一种肠道微生物，通常栖息在健康个体的粘膜表面，作为常驻微生物群的一部分。然而，在易感宿主，特别是住院和/或免疫功能低下的患者中，它可能引起广泛的感染。非生物基质的存在，如中心静脉或导尿管，为念珠菌附着和持久性提供了额外的生态位，特别是通过生物膜发育。此外，念珠菌生物膜对大多数抗真菌药（包括唑类）的敏感性较差。在这里，我们研究了已知在体外、离体和/或体内对不同病原体具有活性的合成杀伤肽（KP）对C.白色念珠菌生物膜与用作阴性对照的乱序肽一起，在不同发育阶段针对念珠菌属生物膜测试KP。一个参考菌株，两种抗氟康唑和两种抗氟康唑敏感的C.使用白色念珠菌临床分离株。KP诱导的C.还分析了白色念珠菌的氧化应激反应和膜通透性。此外，还研究了KP对C.白念珠菌生物膜形成的不同阶段，如细胞粘附，菌丝发育和细胞外基质的生产，涉及的基因进行了评估。我们的研究结果清楚地表明，与KP处理强烈影响的能力，C。白色念珠菌形成生物膜，并显着损害预先形成的成熟生物膜。KP处理导致C.白念珠菌氧化应激反应和膜通透性;生物膜相关基因的表达也显著降低。在所有使用的菌株中观察到相当的抑制作用，无论其对氟康唑的耐药性或敏感性如何。最后，还观察到KP介导的对导管相关C.白色念珠菌生物膜本研究首次提供了KP对C.提示KP可能被认为是治疗和预防生物膜相关的白色念珠菌的潜在新工具。白色念珠菌感染
Candida albicans is a commensal organism, commonly inhabiting mucosal surfaces of healthy individuals, as a part of the resident microbiota. However, in susceptible hosts, especially hospitalized and/or immunocompromised patients, it may cause a wide range of infections. The presence of abiotic substrates, such as central venous or urinary catheters, provides an additional niche for Candida attachment and persistence, particularly via biofilm development. Furthermore, Candida biofilm is poorly susceptible to most antifungals, including azoles. Here we investigated the effects of a synthetic killer peptide (KP), known to be active in vitro, ex vivo and/or in vivo against different pathogens, on C. albicans biofilm. Together with a scrambled peptide used as a negative control, KP was tested against Candida biofilm at different stages of development. A reference strain, two fluconazole-resistant and two fluconazole-susceptible C. albicans clinical isolates were used. KP-induced C. albicans oxidative stress response and membrane permeability were also analysed. Moreover, the effect of KP on transcriptional profiles of C. albicans genes involved in different stages of biofilm development, such as cell adhesion, hyphal development and extracellular matrix production, was evaluated. Our results clearly show that the treatment with KP strongly affected the capacity of C. albicans to form biofilm and significantly impairs preformed mature biofilm. KP treatment resulted in an increase in C. albicans oxidative stress response and membrane permeability; also, biofilm-related genes expression was significantly reduced. Comparable inhibitory effects were observed in all the strains employed, irrespective of their resistance or susceptibility to fluconazole. Finally, KP-mediated inhibitory effects were observed also against a catheter-associated C. albicans biofilm. This study provides the first evidence on the KP effectiveness against C. albicans biofilm, suggesting that KP may be considered as a potential novel tool for treatment and prevention of biofilm-related C. albicans infections.
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影响因子：
3.6
作者：
Leberer, E;Harcus, D;Schröppel, K
通讯作者：
Schröppel, K
影响因子：
4.9
作者：
De Brucker, Katrijn;Delattin, Nicolas;Thevissen, Karin
通讯作者：
Thevissen, Karin
影响因子：
3.7
作者：
Hua, J.;Yamarthy, R.;Diamond, G.
通讯作者：
Diamond, G.
影响因子：
3.1
作者：
Enjalbert, Brice;Rachini, Anna;d'Enfert, Christophe
通讯作者：
d'Enfert, Christophe
影响因子：
--
作者：
Coste, Alix T.;Crittin, Jerome;Sanglard, Dominique
通讯作者：
Sanglard, Dominique