Fabrication of N-acetyl-L-cysteine and L-cysteine functionalized chitosan-casein nanohydrogels for entrapment of hydrophilic and hydrophobic bioactive compounds
Fabrication of N-acetyl-L-cysteine and L-cysteine functionalized chitosan-casein nanohydrogels for entrapment of hydrophilic and hydrophobic bioactive compounds
复制标题
用于包埋亲水性和疏水性生物活性化合物的 N-乙酰基-L-半胱氨酸和 L-半胱氨酸功能化壳聚糖-酪蛋白纳米水凝胶的制备
DOI：
10.1016/j.foodhyd.2019.05.039
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发表时间：
2019-11-01
影响因子：
10.7
通讯作者：
Liu, Boqun
中科院分区：
文献类型：
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作者：
Du, Zhiyang;Liu, Jingbo;Liu, Boqun
在这项工作中，我们提出了自组装纳米水凝胶（NG）系统的控制和持续释放的亲水性以及疏水性生物活性化合物。通过层层静电相互作用合成N-乙酰-L-半胱氨酸（NAC）和L-半胱氨酸（CYS）功能化壳聚糖（CS）和NaOH修饰的酪蛋白（CA），以提高鸡蛋白色衍生肽（EWDP）和姜黄素的生物利用度。动态光散射结果表明，NAC-CS-CA NG和CYS-CS-CA NG具有pH敏感性，最小粒径分别为89和86 nm。NG在室温下储存21天显示出优异的储存稳定性。在不同pH值条件下，NAC-CS-CA NG对EWDP和姜黄素的包封率分别为39 - 67%和51-63%; CYS-CS-CA NG对EWDP和姜黄素的包封率分别为45-58%和48-63%。通过傅立叶变换红外光谱（FTIR）对NG进行了表征，以研究化学相互作用以及评价载药制剂的包封机制。体外释药动力学结果表明，EWDP和姜黄素从NG中的释放分别可延长至56 h和80 h。NGs抑制EWDP和姜黄素在胃介质中的突释，增强EWDP和姜黄素在肠介质中的缓释。目前的研究展示了有前途的新方法，设计的商业生产的封装的生物活性化合物的功能属性改善的递送系统。
In this work, we present self-assembly nanohydrogels (NGs) systems for controlled and sustained release of hydrophilic as well as hydrophobic bioactive compounds. Specifically, N-acetyl-L-cysteine (NAC) and L-cysteine (CYS) functionalized chitosan (CS) and NaOH modified casein (CA) were synthesized by layer-by-layer electrostatic interaction to enhance the bioavailability of egg white derived peptides (EWDP) and curcumin. The NAC-CS-CA NGs and CYS-CS-CA NGs exhibited pH-sensitive properties with the smallest particle size of 89 and 86 nm by dynamic light scattering, respectively. The NGs showed excellent storage stability for 21 days in room temperature. The entrapment efficiency (EE) of EWDP and curcumin ranges from 39 to 67% and 51-63% in NAC-CS-CA NGs, 45-58% and 48-63% in CYS-CS-CA NGs at different pH values. The NGs had been characterized by Fourier transform infrared (FTIR) spectroscopy to investigate chemical interactions as well as to evaluate the entrapment mechanism of the drug-loaded formulations. The in vitro release kinetics of EWDP and curcumin showed prolong drug release up to 56 h and 80 h from the NGs, respectively. Moreover, the NGs inhibited the burst release of EWDP and curcumin at gastric media and enhanced the sustained release at intestinal media. The current study exhibited the promising novel method for designing delivery systems for commercial production of encapsulated bioactive compounds with improved functional attributes.