Biotransformation of thiamine tetrahydrofurfuryldisulfide and its effect on potassium loss in isolated guinea-pig atria.
Biotransformation of thiamine tetrahydrofurfuryldisulfide and its effect on potassium loss in isolated guinea-pig atria.
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硫胺素四氢糠基二硫化物的生物转化及其对离体豚鼠心房钾损失的影响。
DOI：
10.1253/jcj.37.135
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
R. Fugono
中科院分区：
文献类型：
--
作者：
J. Sugimoto;M. Nagata;R. Fugono
在我们之前的论文中，作者报道了在无钾培养基中保存的豚鼠离体心房中钾含量的减少可以通过在培养基中添加四氢呋喃二硫化硫胺素（TTFD）来防止。在本实验中，试图澄清这种药物效应是否由源自TTFD的生物合成硫胺素引起。在心房中观察TTFD的作用，心房先用TTFD处理一次，然后用Locke's溶液冲洗，最后用含低浓度钾离子的培养基处理，不加TTFD。由于这种效应似乎是由来自TTFD的某种生物合成化合物引起的，因此对游离形式和结合形式的硫胺素的数量进行了化学估计。然而，TTFD的作用与生物合成硫胺素的量之间几乎没有直接关系。因此，TTFD的作用被认为是由TTFD在心房中的生物转化产生的。
In our previous paper, the authors had reported that the reduction in potassium content in isolated guinea-pig atria, kept in a potassium-free medium was prevented by thiamine tetrahydrofurfuryldisulfide (TTFD), added to the medium. In the present experiment, the attempt was to clarify whether or not this drug effect was caused by a biosynthetic thiamine originating from the TTFD. The effect of TTFD was observed in atria which had been treated once with TTFD, then washed out with Locke's solution, and finally subjected to a medium containing a low concentration of potassium ions without TTFD. As this effect seemed to be due to some biosynthetic compound originating from the TTFD, the amounts of thiamine in both the free and the bound forms were chemically estimated. A direct relation, however, could scarcely be found between the effect of TTFD and the amount of biosynthetic thiamine. The effect of TTFD was therefore assumed to be produced by the biotransformation of TTFD in the atria.