Association of Streptococcus gallolyticus strains of high and low virulence with the intestinal tract of pigeons
Association of Streptococcus gallolyticus strains of high and low virulence with the intestinal tract of pigeons
复制标题
DOI：
10.1637/6081
复制
发表时间：
2003-07-01
期刊：
影响因子：
1.4
通讯作者：
Haesebrouck, F
中科院分区：
文献类型：
--
作者：
Kimpe, A;Decostere, A;Haesebrouck, F
我们研究了一株高毒力（STR 357）和一株低毒力（STR 598）的溶胆链球菌对鸽子肠道的附着能力。为此，首先对两组6只鸽子进行麻醉，并在十二指肠、空肠、回肠和结肠的起始处结扎。第一组和第二组的鸡肠袢分别注射溶食沙门氏菌STR 357和STR 598。接种后15、30、60 min，每组处死2只鸽子，取肠袢标本进行组织学、免疫组化和电镜检查。高毒力菌株和低毒力菌株都能粘附在肠黏膜上。的确，所有的样本都清楚地显示出大量的球菌状细菌，这些细菌被溶鸡链球菌抗血清染色呈阳性，并排列在肠上皮上。同样，在电镜检查中，在覆盖肠上皮的粘液中可见球菌。其次，定量确定了不同毒力的溶食沙门氏菌菌株与肠道组织的关系。实验方法如上所述。通过对肠段进行10倍连续稀释，测定粘附在肠上皮上的败食链球菌的数量。高毒力菌株和低毒力菌株的黏附菌数无显著差异。
We investigated the ability of a high virulence (STR 357) and a low virulence (STR 598) strain of Streptococcus gallolyticus to attach to the intestinal tract of pigeons. For that purpose, first of all, two groups of six pigeons were anesthetized and ligatures were placed at the beginning of duodenum, jejunum, ileum, and colon. The obtained intestinal loops of the birds of the first and second group were injected with S. gallolyticus strains STR 357 and STR 598, respectively. At 15, 30, and 60 min postinoculation, two pigeons of each group were euthanatized and the various intestinal loops were sampled for histologic, immunohistochemical, and electron microscopic examination. Both the high and low virulence strains were able to adhere to the intestinal mucosa. Indeed, all samples clearly showed numerous coccal-shaped bacteria that stained positively with S. gallolyticus antiserum and were lining up against the intestinal epithelium. Likewise, on electron microscopic examination, cocci were seen in the mucus covering the intestinal epithelium. Second, the association of S. gallolyticus strains of differing virulence with the intestinal tissue was determined quantitatively. Experiments were performed as described above. The number of S. gallolyticus bacteria that adhered to the intestinal epithelium was determined by plating out 10-fold serial dilutions of the segments. No significant differences in the number of adhered bacteria were found between the strains of high and low virulence.