Differences in shoot Na+ accumulation between two tomato species are due to differences in ion affinity of HKT1;2
Differences in shoot Na+ accumulation between two tomato species are due to differences in ion affinity of HKT1;2
复制标题
DOI：
10.1016/j.jplph.2013.12.001
复制
发表时间：
2014-03-15
影响因子：
4.3
通讯作者：
de Boer, Albertus H.
中科院分区：
文献类型：
--
作者：
Almeida, Pedro;de Boer, Gert-Jan;de Boer, Albertus H.
HKT 1在植物Na+稳态中起重要作用。本文报道了耐盐性不同的两个番茄品种：番茄Solanum lycopersicum和番茄Solanum pennellii不同器官中Na+积累的分析。此外，我们与这些差异之间的Na+积累的两个物种的差异，在HKT 1;2运输动力学和HKT 1;2的表达。S. lycopersicum表现出“Na+排斥”行为，而S. pennellii则表现出“Na+包合”行为。SlHKT 1;2表达，与之相反。NaCl处理对SpHKT 1：2的表达有显著影响，尤其是茎中SlHKT 1：2的表达量有很高的增加。SlHKT 1：2启动子-GUS报告基因分析表明，SlHKT 1：2在转化的拟南芥植物的根和芽周围的脉管系统中表达。在本文中，我们提出了两种番茄的HKT 1;2蛋白序列，并提供证据表明，这两个SlHKT 1;2和SpHKT 1;2是Na+转运蛋白。我们的动力学研究表明，SpHKT 1;2，与SlHKT 1;2相比，具有较低的亲和力Na+。SpHKT 1;2的低亲和力与S. pennellii。我们的研究结果表明，重要的是要了解运输特性的HKT 1：2转运蛋白，以提高理解Na+的植物体内平衡。(C)2013年爱思唯尔有限公司。All rights reserved.
HKT1 has been shown to be essential in Na+ homeostasis in plants. In this paper, we report the analysis of Na+ accumulation in different plant organs of two tomato species with contrasting salt tolerances: Solanum lycopersicum and Solanum pennellii. Furthermore, we relate these differences in Na+ accumulation between the two species to the differences in HKT1;2 transport kinetics and HICT1;2 expression. S. lycopersicum showed "Na+ excluder" behaviour, whereas S. pennellii showed "Na+ includer" behaviour. SlHKT1;2 expression, in contrast to. SpHKT1;2 expression showed a significant effect of NaCl treatment, especially stems had a high increase in SlHKT1:2 expression. SlHKT1:2 promoter-GUS reporter gene analysis showed that SlHKT1;2 is expressed in the vasculature surrounding the roots and shoots of transformed Arabidopsis plants. In this paper, we present HKT1;2 protein sequences of both tomato species and provide evidence that both SlHKT1;2 and SpHKT1;2 are Na+ transporters. Our kinetic studies showed that SpHKT1;2, in comparison with SlHKT1;2, had a lower affinity for Na+. This low affinity of SpHKT1;2 correlated with higher xylem Na+ and higher accumulation of Na+ in stems and leaves of S. pennellii. Our findings demonstrate the importance of the understanding of transport characteristics of HKT1:2 transporters to improve the understanding of Na+ homeostasis in plants. (C) 2013 Elsevier GmbH. All rights reserved.