Isolation and molecular characterisation of Achromobacter phage phiAxp-3, an N4-like bacteriophage.
Isolation and molecular characterisation of Achromobacter phage phiAxp-3, an N4-like bacteriophage.
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无色杆菌噬菌体 phiAxp-3（一种 N4 样噬菌体）的分离和分子表征
DOI：
10.1038/srep24776
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发表时间：
2016-04-20
影响因子：
4.6
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Ma Y;Li E;Qi Z;Li H;Wei X;Lin W;Zhao R;Jiang A;Yang H;Yin Z;Yuan J;Zhao X
木糖嗜铬杆菌是一种机会性致病菌，可引起各种医院和社区获得性感染。我们从医院垃圾中分离出phiAxp-3，这是一种感染木霉的类似N4的噬菌体，并对其基因组和生物学特性进行了研究。透射电子显微镜显示，phiAxp-3有一个直径为67 nm的二十面体头部和一个20 nm的非收缩尾巴，具有Podoviridae噬菌体(尾叶病毒目)的特征。PhiAxp-3的突发性空斑大小为9,000个空斑形成单位，潜伏期为80 min，其寄主范围仅限于所测试的35株木霉中的4株。基因组全长72,825 ，末端多馀416个碱基，含80个开放阅读框，与毒力或耐药性无关。基因组序列比较表明，phiAxp-3与JWAlpha和JWDelta无色杆菌噬菌体的关系比与其他N4病毒的更近。利用蛋白质组学技术，我们从纯化的phiAxp-3颗粒中鉴定了25种病毒蛋白。值得注意的是，对宿主细胞表面噬菌体phiAxp-3受体的研究表明，脂多糖是噬菌体phiAxp-3吸附的受体。在一个迫切需要替代疗法来对抗抗生素耐药性细菌的时代，我们的发现促进了对木霉噬菌体的现有认识。
Achromobacter xylosoxidans, an opportunistic pathogen, is responsible for various nosocomial and community-acquired infections. We isolated phiAxp-3, an N4-like bacteriophage that infects A. xylosoxidans, from hospital waste and studied its genomic and biological properties. Transmission electron microscopy revealed that, with a 67-nm diameter icosahedral head and a 20-nm non-contractile tail, phiAxp-3 has features characteristic of Podoviridae bacteriophages (order Caudovirales). With a burst size of 9000 plaque-forming units and a latent period of 80 min, phiAxp-3 had a host range limited to only four A. xylosoxidans strains of the 35 strains that were tested. The 72,825 bp phiAxp-3 DNA genome, with 416-bp terminal redundant ends, contains 80 predicted open reading frames, none of which are related to virulence or drug resistance. Genome sequence comparisons place phiAxp-3 more closely with JWAlpha and JWDelta Achromobacter phages than with other N4 viruses. Using proteomics, we identified 25 viral proteins from purified phiAxp-3 particles. Notably, investigation of the phage phiAxp-3 receptor on the surface of the host cell revealed that lipopolysaccharide serves as the receptor for the adsorption of phage phiAxp-3. Our findings advance current knowledge about A. xylosoxidans phages in an age where alternative therapies to combat antibiotic-resistant bacteria are urgently needed.