ISOLATION AND CHARACTERIZATION OF CALMODULIN GENES FROM XENOPUS-LAEVIS
ISOLATION AND CHARACTERIZATION OF CALMODULIN GENES FROM XENOPUS-LAEVIS
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DOI：
10.1128/mcb.4.3.507
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发表时间：
1984-01-01
影响因子：
5.3
通讯作者：
DAWID, IB
中科院分区：
文献类型：
--
作者：
CHIEN, YH;DAWID, IB
两个来自X的C[互补]DNA。克隆了羊钙调素mRNA。两种cDNA都含有完整的蛋白质编码区和不同长度的非翻译片段。这两个cDNA编码相同的蛋白质，但彼此之间有5%的核苷酸取代。5“和3”非翻译区，在可获得的范围内，在2个cDNA之间高度同源。X.与哺乳动物和鸡的脊椎动物钙调素相同，与电鳗钙调素相比显示1个取代。分离了与2个cDNA克隆同源的基因组DNA序列，并显示其为X中主要的钙调素编码DNA序列。光滑。X. laevis携带2个活性的、非等位的钙调素基因。副艇长显然，乳钙调蛋白基因被至少4个内含子中断。钙调素mRNA的相对浓度估计在不同的胚胎阶段和成人组织，发现变化高达10倍。在卵巢、睾丸和大脑中发现了最高水平的钙调素mRNA。在这3种组织中，2种钙调素基因似乎以大致相等的水平表达。
Two c[complementary]DNA derived from X. laevis calmodulin mRNA were cloned. Both cDNA contain the complete protein-coding region and various lengths of untranslated segments. The 2 cDNA encode an identical protein but differ from each other by 5% nucleotide substitutions. The 5'' and 3'' untranslated regions, to the extent available, are highly homologous between the 2 cDNA. The predicted sequence of X. laevis calmodulin is identical to that of vertebrate calmodulins from mammals and chickens and shows 1 substitution compared with electric eel calmodulin. Genomic DNA sequences homologous to each of the 2 cDNA clones were isolated and were shown to account for the major calmodulin-coding DNA sequences in X. laevis. X. laevis carries 2 active, nonallelic calmodulin genes. The X. laevis calmodulin genes apparently are interrupted by at least 4 introns. The relative concentrations of calmodulin mRNA were estimated in different embryonic stages and adult tissues and found to vary by up to a factor of 10. The highest levels of calmodulin mRNA were found in ovaries, testes and brains. In these 3 tissues, the 2 calmodulin genes appear to be expressed at approximately equal levels.