Low prevalence of antibiotic-resistant gram-negative bacteria isolated from rural south-western Ugandan groundwater
Low prevalence of antibiotic-resistant gram-negative bacteria isolated from rural south-western Ugandan groundwater
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从乌干达西南部农村地下水中分离出的耐抗生素革兰氏阴性细菌的流行率较低
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
M. Roberts
中科院分区：
文献类型：
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作者：
O. Soge;M. A. Giardino;I. Ivanova;A. Pearson;J. Meschke;M. Roberts
本研究的目的是确定从乌干达西南部2个农村的42个不同饮用水源中回收的革兰氏阴性肠道细菌的抗生素耐药模式和特定耐药基因。这些水源地容易受到人类和牲畜活动的污染。检测到的52株分离株中，26株携带抗生素耐药基因，其中25株为氨苄西林耐药，21株携带blaTEM基因，没有分离株携带扩展谱<s:1>内酰胺酶编码基因。12株菌株具有四环素耐药，这些菌株携带1至3种不同的tet基因，以tet(A)基因最为常见。6株分离株携带大环内酯类耐药基因mef(A)和/或大环内酯类耐药基因erm(B)。4个分离株携带sul1基因，4个分离株携带sul1和int1基因，表明存在1类整合子。在本研究中，乌干达分离株的抗生素和多药耐药基因携带率低于预期，携带1类整合子，缺乏编码扩展谱<e:2>的基因。内酰胺酶与非洲临床分离株抗生素耐药携带的比较。
The objective of this study was to determine antibiotic resistance patterns and specific resistance genes in Gram-negative enteric bacteria recovered from 42 different drinking water sources servicing 2 rural villages in south-western Uganda. These water sites were prone to contamination by both human and cattle activity. Of the 52 isolates examined, 26 carried antibiotic resistance genes with 25 being ampicillin resistant, 21 carrying the blaTEM gene, and no isolate carrying genes coding for extended-spectrum â.lactamases. Twelve isolates were tetracycline resistant and these bacteria carried between 1 and 3 different tet genes, with the tet(A) gene the most common. Six isolates carried the macrolide resistance mef(A) and/or the macrolide-lincosamide-streptogramin B resistance erm(B) genes. Four isolates carried the sul1 gene, and 4 isolates carried the sul1 and int1 genes indicating the presence of Class 1 integrons. The Ugandan isolates in this study had lower than expected carriage rates of antibiotic and multi-drug resistance genes, carriage of Class 1 integrons and lacked genes coding for extended-spectrum â.lactamases as compared to antibiotic resistance carriage in clinical African isolates.