The host jasmonic acid pathway regulates the transcriptomic changes of dodder and host plant under the scenario of caterpillar feeding on dodder
The host jasmonic acid pathway regulates the transcriptomic changes of dodder and host plant under the scenario of caterpillar feeding on dodder
复制标题
毛毛虫取食菟丝子场景下宿主茉莉酸途径调控菟丝子及寄主植物的转录组变化
DOI：
10.1186/s12870-019-2161-8
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发表时间：
2019-12-04
影响因子：
5.3
通讯作者：
Wu, Jianqiang
中科院分区：
文献类型：
--
作者：
Qin, Yan;Zhang, Jingxiong;Wu, Jianqiang
背景：菟丝子（菟丝子属，旋花科）物种是专性无叶和无根寄生虫，完全依赖宿主生存。菟丝子自然地将自己嫁接到宿主茎上，形成维管融合，从中获得营养和水分。此外，菟丝子和它们的宿主还交换各种其他分子，包括蛋白质、mRNA和小RNA。这是很可能的维管融合还允许系统信号在植物间的转移和这些系统信号可能会对菟丝子和寄主植物的生理产生深远的影响。植食性胁迫是植物常见的一种生物胁迫。当菟丝子寄生虫受到鳞翅目昆虫的攻击时，菟丝子对毛虫取食的反应以及寄主植物和寄生虫之间是否存在植物间的通讯仍然知之甚少。在这里，野生型（WT）烟草和茉莉酸（JA）生物合成被沉默的烟草品系利用AOC-RNAi技术，研究了斜纹夜蛾幼虫取食对菟丝子及其寄主植物的影响。结果表明，在受毛虫攻击后，AOC-RNAi烟草上生长的菟丝子表现出更少的差异表达基因，尽管烟草植株的基因型对模拟的S.取食斜纹夜蛾诱导了菟丝子体内JA的积累。进一步证明了S.斜纹夜蛾对菟丝子的取食也导致了寄主烟草转录组和防御代谢产物的巨大变化，从而增强了对S.结论：我们的研究结果表明，在毛虫攻击菟丝子的过程中，寄主植物的JA途径是菟丝子和寄主植物适当的转录组反应所必需的。本研究强调了寄主JA途径在调节菟丝子与寄主植物间系统信号传递中的重要性。
Background: Dodder (Cuscuta spp., Convolvulaceae) species are obligate leaf- and rootless parasites that totally depend on hosts to survive. Dodders naturally graft themselves to host stems to form vascular fusion, from which they obtain nutrients and water. In addition, dodders and their hosts also exchange various other molecules, including proteins, mRNAs, and small RNAs. It is very likely that vascular fusion also allows inter-plant translocation of systemic signals between dodders and host plants and these systemic signals may have profound impacts on the physiology of dodder and host plants. Herbivory is a common biotic stress for plants. When a dodder parasite is attacked by lepidopteran insects, how dodder responds to caterpillar feeding and whether there are inter-plant communications between the host plants and the parasites is still poorly understood.Results: Here, wild-type (WT) tobacco and a tobacco line in which jasmonic acid (JA) biosynthesis was silenced (AOC-RNAi) were used as the hosts, and the responses of dodders and their host plants to herbivory by Spodoptera litura caterpillars on the dodders were investigated. It was found that after caterpillar attack, dodders grown on AOC-RNAi tobacco showed much a smaller number of differentially expressed genes, although the genotypes of the tobacco plants did not have an effect on the simulated S. litura feeding-induced JA accumulation in dodders. We further show that S. litura herbivory on dodder also led to large changes in transcriptome and defensive metabolites in the host tobacco, leading to enhanced resistance to S. litura, and the JA pathway of tobacco host is critical for these systemic responses.Conclusions: Our findings indicate that during caterpillar attack on dodder, the JA pathway of host plant is required for the proper transcriptomic responses of both dodder and host plants. This study highlights the importance of the host JA pathway in regulating the inter-plant systemic signaling between dodder and hosts.