Lymphatic Vessel Hypertrophy in Inflamed Human Tonsils
Lymphatic Vessel Hypertrophy in Inflamed Human Tonsils
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DOI：
10.1089/lrb.2009.0026
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发表时间：
2010-01-01
影响因子：
1.4
通讯作者：
Tempero, Richard M.
中科院分区：
文献类型：
--
作者：
Kelley, Philip M.;Tempero, Richard M.
人扁桃体淋巴微血管系统的结构和分子特性对于理解是重要的，因为这些特征可能有助于流体平衡、免疫和肿瘤转移。扁桃体是一个独特的淋巴器官，因为它与上呼吸消化道的内容物密切接触，并且没有可识别的传入神经。常规免疫荧光显微镜显示扁桃体内淋巴管结构的显着程度; LYVE-1阳性淋巴管检测到每个生发中心周围和滤泡之间的边缘区域。高分辨率共聚焦激光扫描免疫荧光显微镜表明，个别淋巴管内皮细胞有一个经典的“橡树叶”的形状和不连续表达的CD31和VE-钙粘蛋白;特征假设与流体和细胞运输。一项比较分析表明，与非炎症扁桃体组织相比，炎症扁桃体组织的淋巴管密度和复杂性显著增加，但血管密度和复杂性没有增加。本研究的结果描述了扁桃体淋巴管系统的空间组织，CD31和VE-钙粘蛋白在人毛细淋巴管中的不连续表达，以及淋巴管形态对炎症反应的变化。
The structural and molecular properties of the human tonsil lymphatic microvascular system are important to understand as these features likely contribute to fluid balance, immunity, and tumor metastasis. The tonsil is a unique lymphoid organ in that it is in intimate contact with the contents of the upper aerodigestive tract and that there are no identifiable afferent lymphatics. Conventional immunofluorescence microscopy demonstrated a remarkable degree of lymphatic vessel architecture within the tonsil; LYVE-1-positive lymphatic vessels were detected around each germinal center and in the marginal regions between the follicles. High resolution confocal laser scanning immunofluoresence microscopy demonstrated that individual lymphatic endothelial cells had a classic 'oak leaf' shape and discontinuous expression of CD31 and VE-cadherin; characteristics hypothesized to be related to fluid and cellular transport. A comparative analysis demonstrated a dramatic increase in lymphatic but not blood vessel density and complexity in inflamed compared to noninflamed tonsil tissue. The results of this study describe the spatial organization of the tonsil lymphatic vasculature, discontinuous expression of CD31 and VE-cadherin in human lymphatic capillaries, and a change in lymphatic vessel morphology in response to inflammation.