Localization of erythropoietin synthesizing cells in murine kidneys by in situ hybridization.
Localization of erythropoietin synthesizing cells in murine kidneys by in situ hybridization.
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DOI：
10.1182/blood.v71.2.524.524
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发表时间：
1988-02
期刊：
影响因子：
20.3
通讯作者：
S. Koury;M. Bondurant;M. Koury
中科院分区：
文献类型：
--
作者：
S. Koury;M. Bondurant;M. Koury
采用原位杂交技术对贫血小鼠肾脏中产生促红细胞生成素（EP）的细胞进行定位。将贫血和非贫血小鼠的肾脏固定并进行石蜡包埋处理。切片用35s标记的RNA探针与编码EP的mRNA互补杂交。一种罕见的，但特殊类型的细胞被强烈标记在贫血肾的皮质。标记的细胞明显呈非球状和非管状。它们在管状基底膜外的位置与间质细胞或毛细血管内皮细胞的亚群一致。
In situ hybridization was used to localize the cells that produce erythropoietin (EP) in anemic murine kidneys. Kidneys from anemic and nonanemic mice were fixed and processed for paraffin embedding. Sections were hybridized with a 35S-labeled RNA probe complementary to mRNA coding for EP. An uncommon, but specific type of cell was intensely labeled in the cortices of anemic kidneys. The labeled cells were clearly nonglomerular and nontubular. Their location outside of the tubular basement membrane was consistent with that of a subset of interstitial cells or capillary endothelial cells.