Multi-window sparse spectral sampling stimulated Raman scattering microscopy
Multi-window sparse spectral sampling stimulated Raman scattering microscopy
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DOI：
10.1364/boe.432177
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发表时间：
2021-10-01
影响因子：
3.4
通讯作者：
Evans, Conor L.
中科院分区：
文献类型：
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作者：
Pence, Isaac J.;Kuzma, Benjamin A.;Evans, Conor L.
受激拉曼散射(SRS)是一种无损、快速的生物和临床标本成像技术，具有无标记的化学特异性。SRS光谱成像通常通过宽带方法进行，或者通过在窄光谱范围内调谐窄带超快光源从而专门针对振动频率来进行。我们展示了一种多窗口稀疏光谱采样SRS((SRS)-R-4)方法，其中一个快速可调的双输出全光纤光学参量振荡器在成像过程中被调谐到超过1400 cm(-1)的特定振动模式。这种方法能够通过扫描全光谱或通过快速调谐到选定的目标频率来收集SRS高光谱图像，免提并自动跨越拉曼光谱的指纹、静音和高波数窗口。我们进一步应用光谱分解和特征选择的计算技术来识别能够区分样本的稀疏拉曼频率子集。在这里，我们应用这种新的方法来监测皮肤中活性药物成分的时空动态变化，这与局部药物产品的给药具有特别的相关性。(C)OSA开放获取出版协议条款下的2021年美国光学学会。
Stimulated Raman scattering (SRS) is a nondestructive and rapid technique for imaging of biological and clinical specimens with label-free chemical specificity. SRS spectral imaging is typically carried out either via broadband methods, or by tuning narrowband ultrafast light sources over narrow spectral ranges thus specifically targeting vibrational frequencies. We demonstrate a multi-window sparse spectral sampling SRS ((SRS)-R-4) approach where a rapidly-tunable dual-output all-fiber optical parametric oscillator is tuned into specific vibrational modes across more than 1400 cm(-1) during imaging. This approach is capable of collecting SRS hyperspectral images either by scanning a full spectrum or by rapidly tuning into select target frequencies, hands-free and automatically, across the fingerprint, silent, and high wavenumber windows of the Raman spectrum. We further apply computational techniques for spectral decomposition and feature selection to identify a sparse subset of Raman frequencies capable of sample discrimination. Here we have applied this novel method to monitor spatiotemporal dynamic changes of active pharmaceutical ingredients in skin, which has particular relevance to topical drug product delivery. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.