Lipoprotein lipase and lipid profiles in plasma and placenta from normal pregnancies compared with patients with intrahepatic cholestasis of pregnancy
Lipoprotein lipase and lipid profiles in plasma and placenta from normal pregnancies compared with patients with intrahepatic cholestasis of pregnancy
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DOI：
10.1016/j.ejogrb.2016.06.019
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发表时间：
2016-08-01
影响因子：
2.6
通讯作者：
Ye, X. L.
中科院分区：
文献类型：
--
作者：
Hao, Z. M.;Ye, Y. F.;Ye, X. L.
目的：分析妊娠期肝内胆汁淤积症(ICP)患者胎盘及血浆中脂蛋白脂酶(LPL)的表达及血脂水平。方法：对30例妊娠期肝内胆汁淤积症患者及30例无并发症的孕妇进行前瞻性研究。用酶联免疫吸附试验检测妊娠28周至38周的血浆LPL水平，每隔4周测定一次，并对数据进行纵向评估。采用免疫组织化学、Western blotting和实时定量聚合酶链式反应检测胎盘LPL的表达和活性。胎盘甘油三酯和总胆固醇水平也进行了分析。结果：两组孕妇血浆LPL浓度均随胎龄增加而升高，但ICP组的升高幅度较小。免疫组织化学显示LPL主要表达于合体滋养层细胞，3，3‘-二氨基联苯胺盐酸盐在正常胎盘组织中的表达明显低于正常胎盘组织(p<；0.01)。妊娠肝内胆汁淤积症患者胎盘组织中LPL蛋白和mRNA的表达均显著低于正常胎盘组织(P<；0.01)。LPL活性在两组间无显著差异。相关分析显示，妊娠肝内胆汁淤积症患者血浆LPL水平与相应的总胆汁酸浓度呈负相关(r=-0.57)。结论：妊娠肝内胆汁淤积症患者胎盘LPL表达减少，母体血浆LPL水平有限升高，血脂谱异常。LPL可能通过参与脂代谢异常参与了肝内胆汁淤积症的发病。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
Objective: To analyse lipoprotein lipase (LPL) expression and lipid levels in placenta and plasma of patients with intrahepatic cholestasis of pregnancy (ICP) and normal pregnancies.Methods: This prospective study included 30 patients with ICP and 30 gestational-age-matched pregnancies without any complications. Enzyme-linked immunosorbent assays were used to investigate plasma LPL levels from 28 weeks of gestation, at 4-weekly intervals, to 38 weeks of gestation, and data were assessed longitudinally. Immunohistochemistry, Western blotting and real-time polymerase chain reaction were used to detect placental LPL expression and activity. Placental triglyceride and total cholesterol levels were also analysed. The clinical data related to ICP and lipid profiles were collected retrospectively.Results: Plasma LPL concentration increased with gestational age in both groups, but the increase was limited in the ICP group. Immunohistochemistry revealed LPL staining mainly in syncytiotrophoblasts, and 3,3'-diamino-benzidine tetrahydrochloride wt% was lower in ICP placenta compared with normal placenta (p < 0.01). LPL protein and mRNA expression in ICP placenta were significantly lower than in normal placenta (p < 0.01). LPL activity was not significantly different in both groups. Correlation analysis indicated that the plasma LPL level was negatively associated with the corresponding concentration of total bile acid (r = -0.57) in the ICP group.Conclusion: Reduced LPL expression in placenta, limited increase in LPL level in maternal plasma, and abnormal lipid profiles were found in patients with ICP. LPL was possibly related to ICP by participating abnormal lipid metabolism. (C) 2016 Elsevier Ireland Ltd. All rights reserved.