IGE-INDUCED HISTAMINE-RELEASE FROM RAT BASOPHILIC LEUKEMIA-CELL LINES - ISOLATION OF RELEASING AND NON-RELEASING CLONES
IGE-INDUCED HISTAMINE-RELEASE FROM RAT BASOPHILIC LEUKEMIA-CELL LINES - ISOLATION OF RELEASING AND NON-RELEASING CLONES
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DOI：
10.1002/eji.1830110410
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
SIRAGANIAN, RP
中科院分区：
文献类型：
--
作者：
BARSUMIAN, EL;ISERSKY, C;SIRAGANIAN, RP
嗜碱性粒细胞释放组胺[HA]是速发型超敏反应的体外模型。大鼠嗜碱性白血病（RBL）细胞可用于研究IgE受体的结构和结合特性。克隆RBL细胞系，并比较各种亚系的染色体数目、IgE介导的HA释放和IgE表面受体。在不同时间从肿瘤开始的细胞系在它们的染色体数目和它们通过IgE介导的反应释放HA的能力方面都不同。RBL-1和III具有. apprx。RBL-II和RBL-IV具有68-73条染色体并显示中等水平的HA释放（释放百分比hivin x [平均值] = 5 ± 1.5），而RBL-II和RBL-IV具有68-73条染色体并显示中等水平的HA释放。2和10 .+-。4、分别）。RBL-IV系的克隆产生了一些亚系，其是优异的HA释放剂（范围39-100%），而一些亚系对IgE介导的HA释放相对难治（< 10%）。这些克隆在染色体数目上没有显著差异。重新克隆的释放线引起差的释放;重新克隆差的释放没有产生良好的释放，表明在文化中的突变漂移是朝着HA释放能力的损失。不同细胞系的IgE受体数量和IgE结合和解离速率相似。该研究未能揭示各种克隆和亚系的IgE受体的显著MW差异，表明释放失败可能不存在于受体中。各种克隆的亚系是表型稳定的，并且优异的HA释放亚系的分离对于HA释放的复杂现象的研究是有用的。
Histamine [HA] release from basophils is an in vitro model for immediate hypersensitivity. The rat basophilic leukemia (RBL) cells are useful for studies of the structure and binding characteristics of the receptor for IgE. The RBL cell lines were cloned and the various sublines compared for their chromosome number, IgE-mediated HA release and for IgE surface receptors. Cell lines started from tumors at different times vary in both their chromosome number and their ability to release HA by an IgE-mediated reaction. RBL-I and III have .apprx. 44 chromosomes and did not respond to an IgE-mediated reaction, whereas RBL-II and RBL-IV have 68-73 chromosomes and showed moderate levels of HA release (percent release .hivin.x [average value] = 5 .+-. 2 and 10 .+-. 4, respectively). The cloning of the RBL-IV line resulted in some sublines which were excellent HA releasers (range 39-100%) and some which were relatively refractory (< 10%) to IgE-mediated HA release. These clones did not differ significantly in chromosome number. Recloning the releasing lines gave rise to poor releasers; the recloning of poor releasers did not produce good releasers indicating that the mutational drift in culture is toward loss of HA-releasing capacity. The IgE receptor number and the rate of IgE association and dissociation were similar for the different cell lines. The study failed to disclose significant MW differences in the IgE receptor for the various clones and sublines indicating that the failure to release probably does not reside in the receptor. The various cloned sublines are phenotypically stable, and the isolation of excellent HA-releasing sublines are useful for studies of the complex phenomenon of HA release.