Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium.
Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium.
复制标题
DOI：
10.1099/ijs.0.024356-0
复制
发表时间：
2011-06
影响因子：
2.8
通讯作者：
Qiliang Lai;Zhiwei Yu;Jianning Wang;Huanzi Zhong;Fengqin Sun;Liping Wang;Baojiang Wang;Z. Shao
中科院分区：
文献类型：
--
作者：
Qiliang Lai;Zhiwei Yu;Jianning Wang;Huanzi Zhong;Fengqin Sun;Liping Wang;Baojiang Wang;Z. Shao
菌株pht-3B(T)是从太平洋富集沉积物中芘降解联合体中分离出来的，该联合体是在筛选多环芳烃降解细菌时收集的。细胞为革兰氏阴性，短杆状，通过鞭毛运动。在0- 7% NaCl和10-41℃条件下观察到生长。该分离物能够将硝酸盐还原为亚硝酸盐，但不能将其还原为氮。结果表明，菌株pht-3B(T)与水生硝化还原菌NL21(T) （16S rRNA基因序列相似性为97.3%）、N. indicus C115(T)（97.1%）、N. basaltis J3(T)（96.8%）和N. kimnyeongensis KY 101(T)（96.7%）亲缘关系最密切。菌株pht-3B(T)与这些参考菌株的DNA-DNA杂交分别显示55.4%、54.4%、28%和42%的DNA-DNA亲缘性。优势脂肪酸为C(19.0)ω8c环（22.6%）和总特征8(由C（18.1)ω7c和/或C(18.1)ω6c组成）；60.4%)。染色体DNA中G+C含量为63 mol%。这些特征与Nitratireductor属成员的特征一致。根据细胞形态、生理、脂肪酸组成、16S rRNA基因序列分析和DNA-DNA亲缘关系分析，该分离物属于Nitratireductor属，但很容易与该属的已知种区分开来。因此，提出了一个新的物种来容纳菌株pht-3B(T)，并建议将其命名为Nitratireductor pacificus sp. 11。型应变为pht-3B(T) (= CCTCC AB 209302(T) = LMG 25541（T) = MCCC 1A01024(T)）。
Strain pht-3B(T) was isolated from a pyrene-degrading consortium of an enriched sediment from the Pacific Ocean, collected during the screening of polycyclic aromatic hydrocarbon-degrading bacteria. Cells were Gram-negative, short rods that were motile by means of flagella. Growth was observed at 0-7 % NaCl and 10-41 °C. The isolate was able to reduce nitrate to nitrite, but not to nitrogen. 16S rRNA gene sequence comparisons showed that strain pht-3B(T) was most closely related to Nitratireductor aquibiodomus NL21(T) (97.3 % 16S rRNA gene sequence similarity), N. indicus C115(T) (97.1 %), N. basaltis J3(T) (96.8 %) and N. kimnyeongensis KY 101(T) (96.7 %). DNA-DNA hybridization between strain pht-3B(T) and these reference strains revealed 55, 54, 28 and 42 % DNA-DNA relatedness, respectively. The dominant fatty acids were C(19 : 0)ω8c cyclo (22.6 %) and summed feature 8 (consisting of C(18 : 1)ω7c and/or C(18 : 1)ω6c; 60.4 %). The G+C content of the chromosomal DNA was 63 mol%. These characteristics were in good agreement with those of members of the genus Nitratireductor. According to cell morphology, physiology, fatty acid composition, 16S rRNA gene sequence analysis and DNA-DNA relatedness, the isolate belonged to the genus Nitratireductor but could be readily distinguished from recognized species of the genus. Therefore a novel species is proposed to accommodate strain pht-3B(T), for which the name Nitratireductor pacificus sp. nov. is proposed. The type strain is pht-3B(T) ( = CCTCC AB 209302(T) = LMG 25541(T) = MCCC 1A01024(T)).