Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry.
Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry.
复制标题
采用液相色谱-电喷雾电离串联质谱法测定溶血磷脂酸和 1-磷酸鞘氨醇。
DOI：
10.1007/7651_2017_55
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Morris,AndrewJ
中科院分区：
文献类型：
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作者：
Kraemer,MariaP;Halder,Suchismita;Smyth,SusanS;Morris,AndrewJ
溶血磷脂酸和鞘氨醇-1-磷酸是生物活性脂类，主要通过细胞表面受体介导的作用来调节不同的细胞和生理过程。这些脂质在多种疾病过程中发挥的作用使参与其合成、失活和信号传递的酶和受体成为药物治疗的诱人靶点。在这一章中，我们描述了使用液相色谱-电喷雾电离串联质谱仪灵敏、准确地定量生物体液中的LPA和S1P水平的方法。
Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles played by these lipids in multiple disease processes make the enzymes and receptors involved in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.