Two-dimensional magnetic droplet manipulation platform for miniaturized bioanalytical applications

Two-dimensional magnetic droplet manipulation platform for miniaturized bioanalytical applications
复制标题

用于小型化生物分析应用的二维磁性液滴操纵平台

DOI:
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发表时间:
2007
期刊:
IEEE/LEOS International Conference on Optical MEMS
影响因子:
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通讯作者:
M. Gijs
M. Gijs
中科院分区:
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文献类型:
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作者:
U. Lehmann;C. Vandevyver;V. K. Parashar;D. DeCourten;M. Gijs

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我们提出了一种新的方法来操作小的液体样品的小型化的生物分析系统。在我们的系统中,微小的液体体积是通过一个可变的磁场和磁性微粒内所含的水液滴的相互作用来操纵。我们的设置使宏观生物分析协议容易转换为芯片实验室格式。在这里,我们提出了一个DNA提取和纯化协议,以及生物活性分子的芯片上的光学检测的实施。在这两种情况下,小型化导致反应和检测时间的减少以及灵敏度的增加。
We present a new approach for the manipulation of small liquid samples for the miniaturization of bioanalytical systems. In our system, minute liquid volumes are manipulated via the interaction of a changeable magnetic field and magnetic microparticles contained within aqueous droplets. Our setup enables the easy conversion of macroscopic bioanalytical protocols into the lab-on-a-chip format. Here we present the implementation of a DNA extraction and purification protocol as well as the on-chip optical detection of bioactive molecules. In both cases the miniaturization results in a decrease of the reaction and detection time as well as an increase in sensitivity.
DOI: 10.1006/abio.2000.4577
发表时间: 2000-08-01
影响因子: 2.9
作者:
Tian, HJ;Hühmer, AFR;Landers, JP
通讯作者: Landers, JP