The Schizosaccharomyces pombe S-phase checkpoint differentiates between different types of DNA damage.
The Schizosaccharomyces pombe S-phase checkpoint differentiates between different types of DNA damage.
复制标题
粟酒裂殖酵母 S 期检查点可区分不同类型的 DNA 损伤。
DOI：
10.1093/genetics/149.4.1729
复制
发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Russell,P
中科院分区：
文献类型：
--
作者：
Rhind,N;Russell,P
我们在粟酒裂殖酵母中发现了一个S期DNA损伤检查点。该检查点依赖于Rad 3，S.哺乳动物ATM/ATR检查点蛋白的粟酒属同源物，和Cds 1。cds 1以前被认为只参与复制检查点。在DNA损伤检查点中需要Cds 1，这表明Cds 1可能是S期检查点的通用靶标。与其他检查站不同，S。粟酒裂殖酵母S期DNA损伤检查点区分不同类型的损伤。紫外线照射引起的碱基修饰可以在G1期和S期修复，这引起了检查点，而γ-照射引起的双链断裂如果在复制前诱导则不能被单倍体细胞修复，这不引起检查点。因为在G2期需要相同的基因来响应UV和γ辐射，所以这种区分可能代表在S期对γ响应的主动抑制。
We have identified an S-phase DNA damage checkpoint in Schizosaccharomyces pombe. This checkpoint is dependent on Rad3, the S. pombe homolog of the mammalian ATM/ATR checkpoint proteins, and Cds1. Cds1 had previously been believed to be involved only in the replication checkpoint. The requirement of Cds1 in the DNA damage checkpoint suggests that Cds1 may be a general target of S-phase checkpoints. Unlike other checkpoints, the S. pombe S-phase DNA damage checkpoint discriminates between different types of damage. UV-irradiation, which causes base modification that can be repaired during G1 and S-phase, invokes the checkpoint, while γ-irradiation, which causes double-stranded breaks that cannot be repaired by a haploid cell if induced before replication, does not invoke the checkpoint. Because the same genes are required to respond to UV- and γ-irradiation during G2, this discrimination may represent an active suppression of the γ response during S-phase.