A new crystal form of large ribosomal subunits from Halobacterium marismortui
A new crystal form of large ribosomal subunits from Halobacterium marismortui
复制标题
来自海产盐杆菌的大核糖体亚基的新晶体形式
DOI：
10.1016/0014-5793(86)81042-0
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发表时间：
1986
期刊：
影响因子：
3.5
通讯作者：
A. Yonath
中科院分区：
文献类型：
--
作者：
M. Shoham;J. Müssig;A. Shevack;T. Arad;H. Wittmann;A. Yonath
用聚乙二醇在结晶混合物中加入1.2-1.7 M KCl的条件下，在19°C下获得了一种新的50 S核糖体亚基的三维晶体。晶体衍射X射线至13 Å，并在同步辐射束中稳定4-8小时。作为薄板的聚落，迄今为止只有两个单元胞边的尺寸，147 × 181 Å，夹角为95°，可以通过X射线晶体学和电子显微镜来确定。通过复杂的播种技术生产更厚晶体的尝试正在进行中。
A new form of three‐dimensional crystals of the 50 S ribosomal subunits fromHalobacterium marismortuihas been obtained at 19°C, using polyethylene glycol in the presence of 1.2–1.7 M KCl in the crystallization mixture. The crystals diffract X‐rays to 13 Å and are stable in the synchrotron radiation beam for 4–8 h. Being aggregates of thin plates, the dimensions of only two unit cell edges, 147 × 181 Å, with an angle of 95°, could so far be determined by both X‐ray crystallography and electron microscopy. Attempts to produce thicker crystals by sophisticated seeding are in progress.