PLU-1 nuclear protein, which is upregulated in breast cancer, shows restricted expression in normal human adult tissues: A new cancer/testis antigen?
PLU-1 nuclear protein, which is upregulated in breast cancer, shows restricted expression in normal human adult tissues: A new cancer/testis antigen?
复制标题
DOI：
10.1002/ijc.10644
复制
发表时间：
2002-10-20
影响因子：
6.4
通讯作者：
Taylor-Papadimitriou, J
中科院分区：
文献类型：
--
作者：
Barrett, A;Madsen, B;Taylor-Papadimitriou, J
PLU-1 基因在乳腺癌和乳腺癌细胞系中以信息水平表达，并在除睾丸外的正常成人组织中显示有限表达。预测的蛋白质序列包含多个结构域，包括 PLU 结构域，该结构域由参与转录和/或发育的其他蛋白质共享。我们开发了一种针对 PLU-1 蛋白 C 末端片段的多克隆抗血清，它与其他家族成员几乎没有同源性。使用抗血清 α-PLU-1C 进行的免疫组织化学分析证实了 PLU-1 的核定位。 α-PLU-1C 还与 PLU-1 的小鼠同源物 (mPlu-1) 发生反应，但不与最接近的家族成员 RBP2 发生反应。使用蛋白质印迹分析，PLU-1 在乳腺癌和乳腺癌细胞系中良好表达，而在一系列正常成人组织中未检测到。我们的结果表明 PLU-1 蛋白可能属于睾丸/癌症抗原类别。 (C) 2002 Wiley-Liss, Inc.
The PLU-1 gene is expressed at the level of message in breast cancers and breast cancer cell lines and shows restricted expression in normal adult tissues with the exception of testis. The predicted protein sequence contains several domains, including the PLU domain, which is shared by other proteins involved in transcription and/or development. We have developed a polyclonal antiserum to a C-terminal fragment of the PLU-1 protein, which shows little homology to other family members. Immunohistochemic analysis with the antiserum alpha-PLU-1C confirmed the nuclear localisation of PLU-1. alpha-PLU-1C also reacted with the mouse homologue of PLU-1 (mPlu-1) but not with the closest family member, RBP2. Using Western blot analysis, PLU-1 was shown to be well expressed in breast cancers and breast cancer cell lines, while it was not detected in a range of normal adult tissues. Our results suggest that the PLU-1 protein may belong to the class of testis/cancer antigens. (C) 2002 Wiley-Liss, Inc.