Role of myeloperoxidase in the luminol-dependent chemiluminescence response of phagocytosing human monocytes.
Role of myeloperoxidase in the luminol-dependent chemiluminescence response of phagocytosing human monocytes.
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髓过氧化物酶在吞噬人类单核细胞的鲁米诺依赖性化学发光反应中的作用。
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
S. Seim
中科院分区：
文献类型：
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作者：
S. Seim
鲁米诺依赖的化学发光（CL）的吞噬反应在体外分化过程中稳步下降，约为初始值的10%，由第四天的文化。在同一时期内观察到单核细胞裂解物的髓过氧化物酶（MPO）活性平行下降，并且发现峰值鲁米诺依赖性CL反应和MPO活性之间存在密切相关性。在体外培养4天期间，平行培养物中吞噬单核细胞的光泽精依赖性CL反应下降至初始值的约85%。化学发光测定鲁米诺或光泽精进行固定量的H2O2或酶促产生的H2O2通量的解决方案。辣根过氧化物酶（HRPO）显着增强鲁米诺依赖的CL，但不是光泽精依赖CL的无细胞系统。当用粗MPO提取物代替HRPO时，获得了类似的结果。尽管这一证据表明，鲁米诺依赖性CL是由过氧化物酶增强，HRPO的测定培养基中加入没有增加鲁米诺依赖性CL反应的四天龄，吞噬单核细胞。
The luminol-dependent chemiluminescence (CL)-response of phagocytosing declined steadily during in vitro differentiation and was approximately 10% of the initial value by the fourth day of culture. A parallel decline in myeloperoxidase (MPO)-activity of monocyte cell lysates was observed during the same period, and a close correlation was found between peak luminol-dependent CL-response and MPO-activity. The lucigenin-dependent CL-response of phagocytosing monocytes in parallel cultures declined to about 85% of the initial value during four days of in vitro culture. Chemiluminescence was determined in solutions of luminol or lucigenin subjected to fixed amounts of H2O2 or enzymatically generated fluxes of H2O2. Horseradish peroxidase (HRPO) markedly enhanced the luminol-dependent CL but not the lucigenin-dependent CL of this cell-free system. Similar results were obtained when a crude MPO extract was substituted for the HRPO. Despite this evidence that luminol-dependent CL is enhanced by peroxidases, addition of HRPO to the assay medium did not increase the luminol-dependent CL-response of four days old, phagocytosing monocytes.