Physical Biochemistry: Principles and Applications
Physical Biochemistry: Principles and Applications
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发表时间：
2000-08
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通讯作者：
D. Sheehan
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作者：
D. Sheehan
前言。第一章绪论。1.1生物分子的特殊化学要求。1.2影响分析物结构和稳定性的因素。1.3生物化学中使用的缓冲系统。1.4量化、单位和数据处理。1.5作为物理生物化学资源的万维网。1.6本卷的目标。参考资料。第2章：色谱学。2.1色谱学原理。2.2色谱中使用的性能参数。2.3色层分析设备。2.4层析模式。2.5开放柱层析法。2.6高效液相色谱(HPLC)。2.7快速蛋白质液相色谱。2.8灌注层析法。2.9膜基色谱系统。2.10样品蛋白质的层析。参考资料。第三章光谱技术。3.1光的本质。3.2电磁频谱。3.3紫外可见吸收光谱学。3.4荧光光谱学。3.5使用平面偏振光的光谱技术。3.6红外光谱分析。3.7核磁共振(核磁共振)谱学。3.8电子自旋共振(ESR)谱。3.9激光。3.10表面等离子体激元共振。参考资料。第四章质谱学。4.1质谱学原理。4.2蛋白质/多肽的质谱学。4.3将MS与其他方法对接。4.4质谱仪在生物化学中的应用。参考资料。第五章电泳法。5.1电泳法原理。5.2非变性电泳法。5.3变性电泳法。5.4 DNA测序中的电泳法。5.5等电聚焦(IEF)。5.6免疫电泳法。5.7核酸琼脂糖凝胶电泳法。5.8脉冲场凝胶电泳。5.9毛细管电泳法。5.10电子印迹程序。5.11电穿孔。参考资料。第六章大分子的三维结构测定。6.1蛋白质折叠问题。6.2用核磁共振测定结构。6.3生物大分子的结晶。6.4晶体的X射线衍射。6.5电子密度图的计算。6.6其他衍射法。6.7 X射线结晶学与多维核磁共振的比较。6.8结构数据库。参考资料。第七章水动力方法。7.1粘度。7.2沉淀物。7.3改变缓冲条件的方法。7.4流式细胞术。参考资料。第八章生物量热法。8.1主要热力学参数。8.2等温滴定量热法。8.3差示扫描量热法。8.4用非量热方法测定热力学参数。参考资料。第九章生物信息学。9.1生物信息学概述。9.2序列数据库。9.3初级结构分析工具。9.4三级结构数据库。9.5三级结构数据库分析和可视化程序。9.6同源建模。参考资料。第十章蛋白质组学。10.1蛋白质组学中的电泳学。10.2蛋白质组学中的质谱学。10.3蛋白质组学中的芯片技术。10.4翻译后修饰蛋白质组学。进一步阅读。参考资料。附录1国际标准单位。附录2傅立叶变换。索引。
Preface. Chapter 1 Introduction. 1.1 Special Chemical Requirements of Biomolecules. 1.2 Factors Affecting Analyte Structure and Stability. 1.3 Buffering Systems Used in Biochemistry. 1.4 Quantitation, Units and Data Handling. 1.5 The Worldwide Web as a Resource in Physical Biochemistry. 1.6 Objectives of this Volume. References. Chapter 2 Chromatography. 2.1 Principles of Chromatography. 2.2 Performance Parameters Used in Chromatography. 2.3 Chromatography Equipment. 2.4 Modes of Chromatography. 2.5 Open Column Chromatography. 2.6 High Performance Liquid Chromatography (HPLC). 2.7 Fast Protein Liquid Chromatography. 2.8 Perfusion Chromatography. 2.9 Membrane-Based Chromatography Systems. 2.10 Chromatography of a Sample Protein. References. Chapter 3 Spectroscopic Techniques. 3.1 The Nature of Light. 3.2 The Electromagnetic Spectrum. 3.3 Ultraviolet/Visible Absorption Spectroscopy. 3.4 Fluorescence Spectroscopy. 3.5 Spectroscopic Techniques Using Plane-Polarized Light. 3.6 Infrared Spectroscopy. 3.7 Nuclear Magnetic Resonance (NMR) Spectroscopy. 3.8 Electron Spin Resonance (ESR) Spectroscopy. 3.9 Lasers. 3.10 Surface Plasmon Resonance. References. Chapter 4 Mass Spectrometry. 4.1 Principles of Mass Spectrometry. 4.2 Mass Spectrometry of Proteins/Peptides. 4.3 Interfacing MS with other Methods. 4.4 Uses of Mass Spectrometry in Biochemistry. References. Chapter 5 Electrophoresis. 5.1 Principles of Electrophoresis. 5.2 Nondenaturing Electrophoresis. 5.3 Denaturing Electrophoresis. 5.4 Electrophoresis in DNA Sequencing. 5.5 Isoelectric Focusing (IEF). 5.6 Immunoelectrophoresis. 5.7 Agarose Gel Electrophoresis of Nucleic Acids. 5.8 Pulsed Field Gel Electrophoresis. 5.9 Capillary Electrophoresis. 5.10 Electroblotting Procedures. 5.11 Electroporation. References. Chapter 6 Three-Dimensional Structure Determination of Macromolecules. 6.1 The Protein-Folding Problem. 6.2 Structure Determination by NMR. 6.3 Crystallization of Biomacromolecules. 6.4 X-Ray Diffraction by Crystals. 6.5 Calculation of Electron Density Maps. 6.6 Other Diffraction Methods. 6.7 Comparison of X-Ray Crystallography with Multi-Dimensional NMR. 6.8 Structural Databases. References. Chapter 7 Hydrodynamic Methods. 7.1 Viscosity. 7.2 Sedimentation. 7.3 Methods for Varying Buffer Conditions. 7.4 Flow Cytometry. References. Chapter 8 Biocalorimetry. 8.1 The Main Thermodynamic Parameters. 8.2 Isothermal Titration Calorimetry. 8.3 Differential Scanning Calorimetry. 8.4 Determination of Thermodynamic Parameters by Non-Calorimetric Means. References. Chapter 9 Bioinformatics. 9.1 Overview of Bioinformatics. 9.2 Sequence Databases. 9.3 Tools for Analysis of Primary Structures. 9.4 Tertiary Structure Databases. 9.5 Programs for Analysis and Visualization of Tertiary Structure Databases. 9.6 Homology Modelling. References. Chapter 10 Proteomics. 10.1 Electrophoresis in Proteomics. 10.2 Mass Spectrometry in Proteomics. 10.3 Chip Technologies in Proteomics. 10.4 Post-Translational Modification Proteomics. Further Reading. References. Appendix 1 SI Units. Appendix 2 The Fourier Transform. Index.