Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation.
Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation.
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DOI：
10.3390/mps4030058
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发表时间：
2021-08-27
影响因子：
2.4
通讯作者：
Jones HN
中科院分区：
文献类型：
--
作者：
Wilson RL;Lampe K;Matushewski BJ;Regnault TRH;Jones HN
开发和实施妊娠治疗的最大挑战之一是在临床相关动物模型中严格测试治疗的能力。豚鼠在研究胎盘、胎儿发育和生殖健康方面具有独特的优势，因为它们在整个妊娠期和出生后都具有与人类相似的发育里程碑。跟踪豚鼠的发情周期是必要的，以确保适当的交配时间，可以通过监测豚鼠阴道膜来执行。在这里，我们描述了一种方法，以有效和准确的时间交配豚鼠，并提供了一个图片表示的变化，在整个发情周期的豚鼠阴道膜。利用这种监测，在5只豚鼠的第一次交配尝试中，怀孕成功率达到100%。这种方法与早期妊娠超声作为确认妊娠的第二种方法一起，为豚鼠提供了一种可靠的定时交配方法。
One of the greatest challenges to the development and implementation of pregnancy therapeutics is the ability to rigorously test treatments in clinically relevant animal models. Guinea pigs offer a unique advantage in studying the placenta, fetal development, and reproductive health as they have similar developmental milestones to humans, both throughout gestation and following birth. Tracking the guinea pig estrus cycle is imperative to ensuring appropriately timed mating and can be performed by monitoring the guinea pig vaginal membrane. Here, we describe a methodology to efficiently and accurately time mate guinea pigs, and provide a picture representation of changes to the guinea pig vaginal membrane throughout the estrus cycle. Utilization of this monitoring enabled a 100% pregnancy success rate on the first mating attempt in a cohort of five guinea pigs. This approach, along with early pregnancy ultrasounds as a secondary method to confirm pregnancy, offers a reliable approach to timed mating in the guinea pig.