Labrenzia suaedae sp nov., a marine bacterium isolated from a halophyte, and emended description of the genus Labrenzia
Labrenzia suaedae sp nov., a marine bacterium isolated from a halophyte, and emended description of the genus Labrenzia
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DOI：
10.1099/ijs.0.052860-0
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发表时间：
2014-04-01
影响因子：
2.8
通讯作者：
Chung, Young Ryun
中科院分区：
文献类型：
--
作者：
Bibi, Fehmida;Jeong, Jae Heon;Chung, Young Ryun
从生长在滩涂的碱蓬植物的灭菌根中分离到一株内生革兰氏染色阴性细菌。该菌株的细胞通过单个极鞭毛运动，并且集落被着色为浅棕色。菌株YC 6927（T）能够在15-37 ℃（最适温度为28-30 ℃）和pH 5.0-10.0（最适pH 7.0-8.0）下生长。该菌株能够在0-9.0%（w/v）的NaCl浓度下生长，在0- 5.0%NaCl下生长最佳。16 S rRNA基因序列分析表明，该菌株与Labrenzia marina G118（T）的同源性最高（97.6%），属于Labrenzia属。菌株YC 6927（T）产生浅棕色类胡萝卜素色素。主要呼吸醌含量为0-10，DNA G+C含量为58.5mol%。菌株YC 6927（T）与近缘菌株之间的DNA DNA相关性在8.2 ± 1.8和20.3 ± 1.5%之间。菌株YC 6927（T）含有作为主要脂肪酸的总特征8（C-18：1 ω 7 c和/或C-18：1 ω 6c）和C-14：0 3-OH，证实了该菌株与Labrenzia属的从属关系。极性脂质由磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰单甲基乙醇胺、一种未知氨基脂质、一种未知磷脂和五种未知脂质组成。根据系统发育分析、生理生化特征和DNA杂交数据，菌株YC 6927（T）应归属于Labrenzia属的一个新种，并建议将其命名为Labrenzia suaedae sp. nov.。模式菌株为YC 6927（T）（=KACC 13772（T）=DSM 22153（T））。本文还提出了一个对Labrenzia属的订正描述。
An endophytic, Gram-staining-negative bacterium was isolated from sterilized roots of a plant, Suaeda maritime, growing on tidal flats. Cells of the strain were motile by means of a single polar flagellum and colonies were pigmented light brown. Strain YC6927(T) was able to grow at 15-37 degrees C (optimum at 28-30 degrees C) and at pH 5.0-10.0 (optimum at pH 7.0-8.0). The strain was able to grow at NaCl concentrations of 0-9.0% (w/v), with optimum growth at 0-5.0% NaCl. Comparison of 16S rRNA gene sequences showed that the strain was a member of the genus Labrenzia, exhibiting the highest similarity to Labrenzia marina mano18(T) (97.6% sequence similarity). Strain YC6927(T) produced light-brown carotenoid pigments. The major respiratory quinone was 0-10 and the DNA G+C content was 58.5 mol%. The DNA DNA relatedness between strain YC6927(T) and closely related strains was between 8.2 +/- 1.8 and 20.3 +/- 1.5%. Strain YC6927(T) contained summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c) and C-14:0 3-OH as major fatty acids, confirming the affiliation of the strain with the genus Labrenzia. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, an unknown aminolipid, an unknown phospholipid and five unknown lipids. On the basis of phylogenetic analysis, physiological and biochemical characterization and DNA DNA hybridization data, strain YC6927(T) should be assigned to a novel species of the genus Labrenzia, for which the name Labrenzia suaedae sp. nov. is proposed. The type strain is YC6927(T) (=KACC 13772(T)=DSM 22153(T)). An emended description of the genus Labrenzia is also proposed.