The Effect of Oral Glucose Tolerance Test on Serum Osteocalcin and Bone Turnover Markers in Young Adults
The Effect of Oral Glucose Tolerance Test on Serum Osteocalcin and Bone Turnover Markers in Young Adults
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DOI：
10.1007/s00223-011-9551-8
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发表时间：
2012-02-01
影响因子：
4.2
通讯作者：
Makitie, O.
中科院分区：
文献类型：
--
作者：
Paldanius, P. M.;Ivaska, K. K.;Makitie, O.
骨钙素（OC）是一种成骨细胞衍生的蛋白质，参与葡萄糖耐量和能量代谢的调节。这种内分泌功能已被认为是通过其羧基化不足的形式发挥作用，这已被证明可以诱导小鼠脂联素，胰岛素和胰岛细胞增殖的表达。此外，胰岛素最近已被证明可以调节骨中OC的生物活性。我们的目的是通过评价标准75 g口服葡萄糖耐量试验（OGTT）对血清OC、羧化OC（cOC）和骨转换标志物（BTM）、I型胶原C末端肽（β CTX-I）和N末端前肽（PINP）以及抗酒石酸酸性磷酸酶5 b（TRACP 5 b）的影响，探讨葡萄糖与骨代谢之间的关系。在血糖正常的年轻成人队列（n = 23，平均年龄23.6岁）中分析在0和120分钟时采集的血清样本。在OGTT期间，观察到所有BTM均显著降低（所有变量P < 0.001）。OC、cOC、β-CTX-I、PINP和TRACP 5 b从0至120 min的中位降低为-32.1%（-37.9至-19.6）、-34.4%（-39.8至-22.2）、-61.4%（-68.5至-53.0）、-26.8%（-33.2至-19.2）和-44.5%（-48.3至-40.2）。OC和cOC的变化之间存在强相关性（r = 0.83，P < 0.001）。PINP的降低与OC的变化相关，而β CTX-I和TRACP 5 b的变化与OC或cOC的降低无关。所观察到的OGTT诱导的骨源性蛋白的变化部分相互独立，并可能由不同的机制介导。
Osteocalcin (OC) is an osteoblast-derived protein implicated in the regulation of glucose tolerance and energy metabolism. This endocrine function has been suggested to be exerted via its undercarboxylated form, which has been shown to induce expression of adiponectin, insulin, and islet cell proliferation in mice. Furthermore, insulin has recently been shown to regulate the biological activity of OC in bone. Our aim was to explore the association between glucose and bone metabolism by evaluating the effect of a standard 75 g oral glucose tolerance test (OGTT) on serum OC, carboxylated OC (cOC) and bone-turnover markers (BTMs) C terminal telopeptide (beta CTX-I) and N terminal propeptide (PINP) of type I collagen and tartrate-resistant acid phosphatase 5b (TRACP5b). Serum samples collected at 0 and at 120 min were analyzed in a cohort of normoglycemic young adults (n = 23, mean age 23.6 years). During OGTT a significant decrease was observed in all BTMs (P < 0.001 for all variables). The median decreases from 0 to 120 min for OC, cOC, beta CTX-I, PINP, and TRACP5b were -32.1% (-37.9 to -19.6), -34.4% (-39.8 to -22.2), -61.4% (-68.5 to -53.0), -26.8% (-33.2 to -19.2), and -44.5% (-48.3 to -40.2), respectively. A strong association between the changes in OC and cOC was observed (r = 0.83, P < 0.001). The decrease in PINP was associated with changes in OC, whereas the changes in beta CTX-I and TRACP5b were not associated with decreases in OC or cOC. The observed OGTT-induced changes in bone-derived proteins were partially independent of each other and potentially mediated by different mechanisms.