Sulfate-reducing bacteria in relation with other potential periodontal pathogens
Sulfate-reducing bacteria in relation with other potential periodontal pathogens
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DOI：
10.1034/j.1600-051x.2001.281210.x
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发表时间：
2001-12-01
影响因子：
6.7
通讯作者：
van der Hoeven, JS
中科院分区：
文献类型：
--
作者：
Langendijk-Genevaux, PS;Grimm, WD;van der Hoeven, JS
背景、目的：口腔硫酸盐还原菌与几种临床类型的牙周炎有关。本横断面研究的目的是比较存在的硫酸盐还原菌（SRB）与其他假定的病原体，包括螺旋体，放线放线杆菌放线菌共生，Bacteroldes，牙龈卟啉单胞菌，和密螺旋体denticola在牙周lesions.Method：牙周炎SRB进行检测富集培养，并与显微镜下的螺旋体计数（n=168）进行比较。针对16 S rRNA的种特异性寡核苷酸探针用于确定A的存在。伴放线菌、牙龈卟啉单胞菌、B.和T.结果：龈下菌斑中SRB的检出率与螺旋体的检出率呈显著正相关（P < 0. 05）。SRB与A的存在呈负相关。放线菌共生菌与此相反，所有的口袋与SRB窝藏T。齿垢毛癣菌或齿垢毛癣菌和B两者。12/14例患者治疗前出现恶心、呕吐。有趣的是，SRB与牙龈卟啉单胞菌的组合发生在治疗前的牙周袋的32%。初步牙周治疗后，这种组合的患病率下降到2%的网站，并在回忆患者的网站为25%。齿生菌B. denticola，B.牙龈卟啉单胞菌和牙龈卟啉单胞菌。这些可疑的病原体形成一个复杂的强烈相关的破坏性牙周炎。
Background, aims: Oral sulfate-reducing bacteria are involved in several clinical categories of periodontitis. The aim of this cross-sectional study was to compare the presence of sulfate-reducing bacteria (SRB) with other putative pathogens including spirochetes, Actinobacillus actinomycetemcomitans, Bacteroldes forsythus, Porphyromonas gingivalis, and Treponema denticola in periodontal lesions.Method: Periodontal SRB were detected by enrichment culture and compared with a microscopic spirochete count (n=168). Species-specific oligonucleotide probes directed against the 16S rRNA were employed to determine the presence of A. aetinomycetemncomitans, P. gingivalis, B. forsythus, and T. denticola (n=55).Results: A significant positive correlation was observed between the presence of SRB and the proportions of spirochetes in subgingival plaque, although the 2 bacterial groups also occurred separately. SRB tended to be negatively correlated with the presence of A. actinonzycetemcomitans. In contrast, all pockets with SRB harbored either T. denticola, or both T denticola and B. forsythus (12/14) before therapy. Interestingly, the combination of SRB with P. gingivalis occurred in 32% of the periodontal pockets before treatment. After initial periodontal therapy, the prevalence of this combination was reduced to 2% of the sites, and to 25% of the sites in recall patients.Conclusion: The presence of SRB was positively correlated with T. denticola, B. forsythus, and P. gingivalis in periodontal lesions. These suspected pathogens form a complex strongly associated with destructive periodontitis.