Requirement of neural activity for the maintenance of dopaminergic neurons in rat midbrain slice cultures
Requirement of neural activity for the maintenance of dopaminergic neurons in rat midbrain slice cultures
复制标题
DOI：
10.1016/s0304-3940(01)01570-1
复制
发表时间：
2001-03-16
影响因子：
2.5
通讯作者：
Akaike, A
中科院分区：
文献类型：
--
作者：
Katsuki, H;Takenaka, C;Akaike, A
用L钙通道阻滞剂尼卡地平(3-10微米)或维拉帕米(10微米)慢性处理器官型中脑片培养18天后，多巴胺能神经元数量急剧减少。电压依赖性钠通道阻滞剂河豚毒素(1um)也能有效地减少多巴胺能神经元的数量。同时应用Forskolin(20um)或二丁基环AMP(1 MM)可抵消尼卡地平和河豚毒素的作用。这些结果表明，中脑脑片培养中自发的神经元活动，导致钙离子通过L型钙通道内流，维持细胞内环磷酸腺苷水平，是维持多巴胺能神经元所必需的。(C)2001年，爱思唯尔科学爱尔兰有限公司出版。
Chronic treatment of organotypic midbrain slice cultures with L-type Ca2+ channel blocker nicardipine (3-10 muM) or verapamil (10 muM) for 18 days resulted in a drastic decrease in the number of dopaminergic neurons. A voltage-dependent Na+ channel blocker tetrodotoxin (1 muM) was also effective in decreasing the number of dopaminergic neurons. Concurrent application of forskolin (20 muM) or dibutyryl cyclic AMP (1 mM) counteracted the effects of nicardipine and tetrodotoxin. These results suggest that spontaneous neuronal activity within midbrain slice cultures, causing Ca2+ influx through L-type Ca2+ channels that maintains intracellular cyclic AMP levels, is required for the maintenance of dopaminergic neurons. (C) 2001 Published by Elsevier Science Ireland Ltd.