Effect of Mono and Di-rhamnolipids on Biofilms Pre-formed by Bacillus subtilis BBK006.
Effect of Mono and Di-rhamnolipids on Biofilms Pre-formed by Bacillus subtilis BBK006.
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DOI：
10.1007/s00284-016-1046-4
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发表时间：
2016-08
影响因子：
2.6
通讯作者：
Martin PJ
中科院分区：
文献类型：
--
作者：
De Rienzo MA;Martin PJ
已知基于嗜热细菌生理学的不同微生物抑制策略对生物膜群落的生长具有有限的功效。这一问题可能会因耐药临床菌株的出现而加剧。由于生物表面活性剂具有分散微生物生物膜的潜力，因此值得在临床和卫生领域重新引起人们的兴趣。在这项工作中，我们探讨了枯草芽孢杆菌BBK 006生物膜的各个方面，并研究了生物衍生的表面活性剂（鼠李糖脂）对枯草芽孢杆菌BBK 006产生的微生物生物膜的破坏或抑制的贡献。评价了铜绿假单胞菌ATCC 9027产生的单鼠李糖脂（Rha-C10-C10）和泰国伯克霍尔德氏菌E264产生的二鼠李糖脂（Rha-Rha-C14-C14）以及磷酸盐缓冲盐水破坏枯草芽孢杆菌BBK 006生物膜的能力。枯草芽孢杆菌BBK 006产生的生物膜对泰国伯克霍尔德氏菌产生的双鼠李糖脂（0.4 g/L）比铜绿假单胞菌ATCC 9027产生的单鼠李糖脂（0.4 g/L）更敏感。鼠李糖脂是生物产生的化合物，对人类使用安全。这使它们成为新一代细菌分散剂的理想候选物，并可用作现有微生物抑制或根除策略的佐剂。
Different microbial inhibition strategies based on the planktonic bacterial physiology have been known to have limited efficacy on the growth of biofilms communities. This problem can be exacerbated by the emergence of increasingly resistant clinical strains. Biosurfactants have merited renewed interest in both clinical and hygienic sectors due to their potential to disperse microbial biofilms. In this work, we explore the aspects of Bacillus subtilis BBK006 biofilms and examine the contribution of biologically derived surface-active agents (rhamnolipids) to the disruption or inhibition of microbial biofilms produced by Bacillus subtilis BBK006. The ability of mono-rhamnolipids (Rha–C10–C10) produced by Pseudomonas aeruginosa ATCC 9027 and the di-rhamnolipids (Rha–Rha–C14–C14) produced by Burkholderia thailandensis E264, and phosphate-buffered saline to disrupt biofilm of Bacillus subtilis BBK006 was evaluated. The biofilm produced by Bacillus subtilis BBK006 was more sensitive to the di-rhamnolipids (0.4 g/L) produced by Burkholderia thailandensis than the mono-rhamnolipids (0.4 g/L) produced by Pseudomonas aeruginosa ATCC 9027. Rhamnolipids are biologically produced compounds safe for human use. This makes them ideal candidates for use in new generations of bacterial dispersal agents and useful for use as adjuvants for existing microbial suppression or eradication strategies.