Taxonomic status of Ixodes neotomae and I. spinipalpis (Acari: Ixodidae) based on mitochondrial DNA evidence.
Taxonomic status of Ixodes neotomae and I. spinipalpis (Acari: Ixodidae) based on mitochondrial DNA evidence.
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基于线粒体 DNA 证据的 Ixodes neotomae 和 I. spinipalpis（螨虫：Ixodidae）的分类地位。
DOI：
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发表时间：
1997
影响因子：
2.1
通讯作者：
W. Black
中科院分区：
文献类型：
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作者：
D. E. Norris;J. Klompen;J. E. Keirans;R. Lane;J. Piesman;W. Black
刺须硬蜱 Hadwen & Nuttall 和 I. neotomae Cooley 是北美洲西部伯氏疏螺旋体 (Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner) 的地方性传播媒介。类群在寄主偏好、栖息地和形态方面存在重叠。对不同类群之间的线粒体 DNA 进行比较，以测试生殖隔离。线粒体 16S 核糖体 DNA 基因的 300 bp 区域在 28 个 Neotomae 样本和 149 个 I. spinipalpis 样本中被扩增。使用单链构象多态性分析对这些产物进行序列变异筛选，并检测到9个单倍型。单倍型频率因类群而异；然而，香农多样性分析表明，大多数变异出现在每个分类单元内的集合之间，并且没有独特的单倍型来表征任一分类单元。以太平洋硬蜱 Cooley & Kohls 和硬蜱 Ixodes jellisoni Cooley & Kohls 作为外群，对 18 个序列（代表 9 个单倍型中每一个的复制）进行了系统发育分析。发现了包含 I. neotomae 和 I spinipalpis 的进化枝的强单系支持，并且在该进化枝内没有仅包含任一分类单元的谱系。这些结果反对将 I. neotomae 和 I. spinipalis 作为不同的物种进行处理。
Ixodes spinipalpis Hadwen & Nuttall and I. neotomae Cooley are enzootic vectors of Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner in western North America. The taxa overlap in host preference, habitat, and morphology. Mitochondrial DNA was compared between the taxa to test for reproductive isolation. A 300-bp region of the mitochondrial 16S ribosomal DNA gene was amplified in 28 specimens of I. neotomae and 149 specimens of I. spinipalpis. These products were screened for sequence variation using single-strand conformation polymorphism analysis, and 9 haplotypes were detected. Haplotype frequencies varied between taxa; however, Shannon diversity analysis indicated that most variation arose among collections within each taxon, and no unique haplotypes characterized either one. Phylogenetic analysis of 18 sequences, representing a replicate of each of the 9 haplotypes, was performed with Ixodes pacificus Cooley & Kohls and Ixodes jellisoni Cooley & Kohls as outgroups. Strong monophyletic support was found for a clade containing I. neotomae and I spinipalpis and within this clade no lineages comprised exclusively either taxon. These results argue against treatment of I. neotomae and I. spinipalpis as distinct species.