Zebrafish circadian clocks: cells that see light.
Zebrafish circadian clocks: cells that see light.
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DOI：
10.1042/bst20050962
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发表时间：
2005-10
影响因子：
3.9
通讯作者：
T. Tamai;A. Carr;D. Whitmore
中科院分区：
文献类型：
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作者：
T. Tamai;A. Carr;D. Whitmore
在生物钟组织的经典观点中，大多数生物体的日常节律被认为是由一个中枢的“主”起搏器调节的，该起搏器通常位于动物的神经结构中。然而，随着在斑马鱼、哺乳动物细胞系和最近的哺乳动物组织中进行的实验结果，这种观点已经改变为现在被视为高度分散的时钟组织的观点。很明显，时钟存在于各种动物的外周组织中，如果蝇、斑马鱼和哺乳动物。就果蝇和斑马鱼而言，这些组织也直接对光有反应。这种光敏感度和直接时钟携带能力也适用于斑马鱼细胞系和早期胚胎。使用含有时钟基因启动子的发光报告细胞系驱动荧光素酶的表达和单细胞成像技术，我们已经能够展示每个细胞如何通过转换到共同的相位来快速响应单个光脉冲，相当于清晨。这种直接的光敏感性可能与这些细胞需要光来激活参与DNA修复的基因的转录有关。同样清楚的是，斑马鱼的生物钟调节着细胞周期的时间，证明了这种光敏感度和日常节律性对斑马鱼生物学的广泛影响。
In the classical view of circadian clock organization, the daily rhythms of most organisms were thought to be regulated by a central, 'master' pacemaker, usually located within neural structures of the animal. However, with the results of experiments performed in zebrafish, mammalian cell lines and, more recently, mammalian tissues, this view has changed to one where clock organization is now seen as being highly decentralized. It is clear that clocks exist in the peripheral tissues of animals as diverse as Drosophila, zebrafish and mammals. In the case of Drosophila and zebrafish, these tissues are also directly light-responsive. This light sensitivity and direct clock entrainability is also true for zebrafish cell lines and early-stage embryos. Using luminescent reporter cell lines containing clock gene promoters driving the expression of luciferase and single-cell imaging techniques, we have been able to show how each cell responds rapidly to a single light pulse by being shifted to a common phase, equivalent to the early day. This direct light sensitivity might be related to the requirement for light in these cells to activate the transcription of genes involved in DNA repair. It is also clear that the circadian clock in zebrafish regulates the timing of the cell cycle, demonstrating the wide impact that this light sensitivity and daily rhythmicity has on the biology of zebrafish.