Immunohistochemical expression patterns of AP2α and AP2γ in the developing fetal human breast
Immunohistochemical expression patterns of AP2α and AP2γ in the developing fetal human breast
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DOI：
10.1111/j.1365-2559.2007.02887.x
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发表时间：
2007-12-01
期刊：
影响因子：
6.4
通讯作者：
Knoepfle, G.
中科院分区：
文献类型：
--
作者：
Friedrichs, N.;Steiner, S.;Knoepfle, G.
目的：AP2α(TFAP2A)和AP2γ(TFAP2G)转录因子参与调控正常乳腺上皮和乳腺癌的增殖、分化和凋亡。本研究的目的是结合其他相关标记，对TFAP2A和TFAP2G在发育中的胎儿乳房原基中的表达模式进行综合分析。方法和结果：检测了60例14周至5月龄的胎儿和1例婴儿乳腺标本。原发乳房突起/乳头基底层细胞表达TFAP2A(第14周)，随后是细胞角蛋白(CK)5共表达(第17周)。次生突起的萌发以HER-2+侵入细胞为特征。用表达TFAP2G/HER-1的肌上皮前体细胞对初生乳管芽进行排列(19周)。TFAP2A/CK18+细胞和TFAP2G/平滑肌肌动蛋白阳性肌上皮细胞的成熟从末端芽开始，从远端到近端进行(22周)。CK5+祖细胞和CK5/TFAP2A或CK5/TFAP2G共表达的中间腺细胞或肌上皮细胞在胎儿乳房末端芽中均有表达。结论：AP2转录因子在胎儿乳房原基形成过程中可能通过调节表皮生长因子受体，在启动和协调萌芽和分支过程中起决定性作用。
Aims: AP2 alpha (TFAP2A) and AP2 gamma (TFAP2G) transcription factors have been implicated in the control of proliferation, differentiation and apoptosis of normal breast epithelium and in breast cancer. The aim of this study was to provide a comprehensive analysis of the expression patterns of TFAP2A and TFAP2G in the developing fetal breast anlage with other relevant markers.Methods and results: Sixty fetal and one infant human breast specimens from 14 weeks of gestational age to 5 months old were examined. The primary breast outgrowth/nipple showed TFAP2A expression by the basal cells (week 14), followed later by cytokeratin (CK) 5 co-expression (week 17). Sprouting of the secondary outgrowths was characterized by HER-2+ invading cells. Preliminary ductal buds were lined by TFAP2G/HER-1-expressing myoepithelial precursors (week 19). Maturation of TFAP2A/CK18+ epithelia and TFAP2G/smooth muscle actin-positive myoepithelia proceeded in a distal-to-proximal manner beginning in the terminal end buds (week 22). CK5+ progenitor cells and CK5/TFAP2A or CK5/TFAP2G co-expressing intermediary glandular or myoepithelial cells were found in the terminal end buds of neonatal fetal breast tissue.Conclusions: AP2 transcription factors may play decisive pacemaker roles in initiating and coordinating budding and branching processes during formation of the fetal breast anlage, possibly via modulation of an epidermal growth factor receptor.