Complementary methods for contact hypersensitivity (CHS) evaluation in mice
Complementary methods for contact hypersensitivity (CHS) evaluation in mice
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DOI：
10.1016/j.jim.2012.11.004
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发表时间：
2013-01-01
影响因子：
2.2
通讯作者：
Szczepanik, Marian
中科院分区：
文献类型：
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作者：
Zemelka-Wiacek, Magdalena;Majewska-Szczepanik, Monika;Szczepanik, Marian
接触超敏反应（CHS）是一种可以在小鼠身上进行研究的变应性接触性皮炎（ACD）实验模型。对于CHS反应，小鼠通过在剃光的腹部和胸部皮肤上涂上活性半抗原，如1-氟-4,6-二硝基苯（DNFB）来免疫。随后，用稀释的半抗原刺激耳朵，引发“超敏”耳肿反应，这种反应可以用千分尺测量。在本文中，我们提出了可用于评估小鼠CHS的补充方法，包括：耳重、血管通透性、髓过氧化物酶（MPO）活性、耳提取物中的ifn - γ浓度以及耳穴淋巴结细胞（ELNC）产生的ifn - γ。增殖试验、ELISA法测定皮肤引流淋巴结细胞的ifn - γ生成量、流式细胞术测定ifn - γ (+) TCR α β CD8细胞对CHS的生化评价也可以得到支持。(c) 2012 Elsevier B.V.版权所有
Contact hypersensitivity (CHS) is an experimental model of allergic contact dermatitis (ACD) that can be studied in mice. For CHS responses, mice are immunized by painting with a reactive hapten, such as 1-fluoro-4,6-dinitrobenzene (DNFB), on the shaved abdominal and chest skin. Subsequently, the ears are challenged with diluted hapten, eliciting 'hypersensitive' ear-swelling reactions, which can be measured with a micrometer. In this manuscript we present complementary methods that can be used to evaluate CHS in mice that include: ear weight vascular permeability, myeloperoxidase (MPO) activity, IFN-gamma concentration in ear extracts and also IFN-gamma production by auricular lymph node cells (ELNC). The biochemical evaluation of CHS can be also supported by proliferation assay, measurement of IFN-gamma production by skin-draining lymph node cells employing ELISA test and by evaluation of IFN-gamma(+) TCR alpha beta CD8 cells with the use of flow cytometry. (c) 2012 Elsevier B.V. All rights reserved.