Induction of morphologically abnormal sperm in rats exposed to O-Xylene.
Induction of morphologically abnormal sperm in rats exposed to O-Xylene.
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DOI：
10.3109/01485018308987487
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发表时间：
1983-12
期刊：
影响因子：
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通讯作者：
W. J. Washington;R. Murthy;A. Doye;K. Eugène;D. Brown;I. Bradley
中科院分区：
文献类型：
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作者：
W. J. Washington;R. Murthy;A. Doye;K. Eugène;D. Brown;I. Bradley
对精子畸形的分析可以作为一种快速确定化学物质的诱变潜力以及化学物质对整个动物的毒性潜力的方法。采用精子畸形试验检测邻二甲苯的潜在致突变性。给10-16周龄SD大鼠(Ip)注射0.5和1.5ml/kg玉米油中的邻二甲苯。治疗五周后，动物被处死，并检查精子的形态异常。观察到了几种形态异常的精子类型，其中包括无定形头部和香蕉状头部的精子。在20-24摄氏度的室温下饲养的动物，与对照组相比，畸形精子的数量没有显著增加。在IP注射0.5ml/kg邻二甲苯后，在24至30摄氏度的温度下饲养的大鼠显示异常精子显著增加。
An analysis of sperm abnormalities can be used as a rapid method to determine the mutagenic potential of chemical agents as well as the toxic potential of chemicals on the whole animal. O-Xylene was investigated for its potential mutagenicity by the sperm abnormality assay. Sprague Dawley rats 10-16 weeks old were injected intraperitoneally (ip) with 0.5 and 1.5 ml/kg body weight of o-xylene in corn oil. Five weeks after treatment, animals were sacrificed and sperm examined for morphological abnormalities. Several morphologically abnormal sperm types were observed; among these were those with amorphous heads, and banana-like heads. Animals housed at room temperatures of 20-24 degrees C showed no significant increase in abnormal sperm over the control. Rats housed at temperatures between 24 and 30 degrees C after ip injection with 0.5 ml/kg o-xylene showed a significant increase in abnormal sperm.